携带鸡卵类粘蛋白和溶菌酶信使核糖核酸互补脱氧核糖核酸的重组质粒的分离。
Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.
作者信息
Buell G N, Wickens M P, Carbon J, Schimke R T
出版信息
J Biol Chem. 1979 Sep 25;254(18):9277-83.
PMID:383714
Abstract
A large library of hen oviduct cDNA-pCR1 recombinant plasmids has been established in Escherichia coli X1776. From this library, ovomucoid cDNA and lysozyme cDNA-bearing plasmids have been identified. One of these plasmids, pMu7, yielded the sequence of the 3'-untranslated region of ovomucoid mRNA.
摘要
在大肠杆菌X1776中已建立了一个庞大的鸡输卵管cDNA-pCR1重组质粒文库。从该文库中，已鉴定出携带卵类粘蛋白cDNA和溶菌酶cDNA的质粒。其中一个质粒pMu7，给出了卵类粘蛋白mRNA 3'非翻译区的序列。
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