Meng Zhuo, Wang Haijuan, Jia Chongyi, Chen Guihua, Zhao Mingmin
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bayannur Agriculture and Animal Husbandry Bureau (Rural Revitalization Bureau), Bayannur City 015000, Mongolia.
Life (Basel). 2025 Apr 14;15(4):642. doi: 10.3390/life15040642.
Lysozyme is an enzyme that hydrolyzes bacterial cell walls, which is functional for destroying the integrity of bacteria, enhancing the activity of immune cells, participating in immune signal transmission, helping to maintain the micro-ecological balance of the gastrointestinal tract, etc. Egg white lysozyme (EWL), as one of the typical representatives of lysozyme, is the most widely used enzyme in production so far, and is also one of the most complex structures of lysozyme. EWL also helps protect plants from fungal and bacterial diseases. Here, we report the effect of EWL on infections from plant viruses. The EWL gene was cloned and characterized. The EWL protein sequence analysis identified a conserved domain of lysozyme activity and the sharing of a 100% identical EWL protein from the lysozyme. Then, the EWL gene was cloned into the plant expression vector pEAQ-HT-DEST3 and transiently expressed in . We found that EWL expression in significantly contributed to infections by the turnip mosaic virus (TuMV) but not by the tobacco mosaic virus (TMV). Plants that transiently expressed EWL showed an obvious increase in resistance to . Our results suggested a new research point for the application of EWL on plant pathogen infections.
溶菌酶是一种能水解细菌细胞壁的酶，其功能包括破坏细菌的完整性、增强免疫细胞的活性、参与免疫信号传递以及有助于维持胃肠道的微生态平衡等。蛋清溶菌酶（EWL）作为溶菌酶的典型代表之一，是目前生产中应用最广泛的酶，也是溶菌酶中结构最为复杂的一种。EWL还有助于保护植物免受真菌和细菌病害。在此，我们报道了EWL对植物病毒感染的影响。对EWL基因进行了克隆和表征。EWL蛋白序列分析确定了一个溶菌酶活性保守结构域，且与溶菌酶有100%相同的EWL蛋白。然后，将EWL基因克隆到植物表达载体pEAQ-HT-DEST3中并在……中瞬时表达。我们发现EWL在……中的表达显著促进了芜菁花叶病毒（TuMV）的感染，但对烟草花叶病毒（TMV）的感染没有促进作用。瞬时表达EWL的植物对……的抗性明显增强。我们的结果为EWL在植物病原体感染方面的应用提出了一个新的研究点。
