Zhang Wei, Jin Can, Zhang Shucheng, Wu Linlin, Li Bohan, Shi Meimei
Department of Critical Care Medicine, The People's Hospital of Leshan, Leshan City, Sichuan Province, 614008, China.
Department of Critical Care Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Heliyon. 2024 Feb 2;10(3):e25711. doi: 10.1016/j.heliyon.2024.e25711. eCollection 2024 Feb 15.
The potential effect of removing danger-associated molecular patterns (DAMPs) from gut lymph on reducing acute lung injury (ALI) induced by gut ischemia-reperfusion injury (GIRI) is uncertain. This study aimed to investigate whether gut lymph purification (GLP) could improve GIRI-induced acute lung injury in rats by clearing danger-associated molecular patterns.
Rats were divided into four groups: Sham, GIRI, GIRI + gut lymph drainage (GLD), and GIRI + GLP. After successful modeling, lung tissue samples were collected from rats for hematoxylin-eosin (HE) staining and detection of apoptotic indexes. We detected the DAMPs levels in blood and lymph samples. We observed the microstructure of AEC Ⅱ and measured the expression levels of apoptosis indexes.
The GIRI group showed destruction of alveolar structure, thickened alveolar walls, and inflammatory cell infiltration. This was accompanied by significantly increased levels of high mobility group protein-1 (HMGB-1) and Interleukin-6 (IL-6), while reduced levels of heat shock protein 70 (HSP 70) and Interleukin-10 (IL-10) in both lymph and serum. In contrast, the lung tissue damage in the GIRI + GLP group was significantly improved compared to the GIRI group. This was evidenced by a reduction in the expression levels of HMGB-1 and IL-6 in both lymph and serum and an increase in HSP 70 and IL-10 levels. Additionally, organelle structure of AEC II was significantly improved in the GIRI + GLP group compared to the GIRI group.
GLP inhibits inflammation and cell apoptosis in GIRI-induced ALI by blocking the link between DAMPs and mononuclear phagocytes, reducing the severity of ALI.
从肠道淋巴中清除危险相关分子模式（DAMPs）对减轻肠道缺血再灌注损伤（GIRI）所致急性肺损伤（ALI）的潜在作用尚不确定。本研究旨在探讨肠道淋巴净化（GLP）是否能通过清除危险相关分子模式来改善GIRI诱导的大鼠急性肺损伤。
将大鼠分为四组：假手术组、GIRI组、GIRI + 肠道淋巴引流（GLD）组和GIRI + GLP组。成功建模后，采集大鼠肺组织样本进行苏木精-伊红（HE）染色及凋亡指数检测。检测血液和淋巴样本中DAMPs水平。观察Ⅱ型肺泡上皮细胞（AEC Ⅱ）的微观结构并测量凋亡指数的表达水平。
GIRI组出现肺泡结构破坏、肺泡壁增厚及炎性细胞浸润。同时，淋巴液和血清中高迁移率族蛋白1（HMGB - 1）和白细胞介素6（IL - 6）水平显著升高，而热休克蛋白70（HSP 70）和白细胞介素10（IL - 10）水平降低。相比之下，GIRI + GLP组的肺组织损伤较GIRI组明显改善。这表现为淋巴液和血清中HMGB - 1和IL - 6表达水平降低，HSP 70和IL - 10水平升高。此外，与GIRI组相比，GIRI + GLP组AEC II的细胞器结构明显改善。
GLP通过阻断DAMPs与单核吞噬细胞之间的联系，抑制GIRI诱导的ALI中的炎症和细胞凋亡，减轻ALI的严重程度。
