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半工业超声辅助提取葡萄籽蛋白质。

Semi-industrial ultrasound-assisted extraction of grape-seed proteins.

机构信息

Department of Drug Science and Technology, University of Turin, Turin, Italy.

出版信息

J Sci Food Agric. 2024 Aug 15;104(10):5689-5697. doi: 10.1002/jsfa.13395. Epub 2024 Mar 21.

Abstract

BACKGROUND

To manage industrial waste in accordance with the circular bioeconomy concept it is sometimes necessary to handle grape seeds, an abundant by-product of the wine-making process. This study presents a process based on ultrasound technology for the extraction of grape-seed proteins, due to their nutritional and techno-functional properties. The protein content of extracts obtained under silent and lab-scale conditions was compared with that obtained under semi-industrial ultrasound conditions, and the chemical composition (carbohydrates, total phenols, and lipids) and the elemental profiles of the final, up-scaled downstream extracts were characterized.

RESULTS

This work found that the maximum amount of protein in the final product was 378.31 g.kg of the extract. Chemical characterization revealed that each 1 kg of extract had an average content of 326.19 g gallic acid equivalent as total phenols, 162.57 g glucose equivalent as carbohydrates, and 382.76 g of lipophilic compounds. Furthermore, when the extract was checked for hazardous elements, none were found in levels that could be considered a risk for human health.

CONCLUSION

The proposed semi-industrial strategy has the potential to contribute greatly to the valorization of grape seeds through the preparation of a protein-rich extract that can be used as an alternative to synthetic wine stabilizers and for the development of novel food and nutraceutical products. © 2024 Society of Chemical Industry.

摘要

背景

为了按照循环生物经济的理念来处理工业废物,有时需要处理葡萄种子,这是酿酒过程中的一种丰富的副产品。本研究提出了一种基于超声技术提取葡萄籽油的工艺,因为其具有营养价值和技术功能特性。比较了在无声和实验室规模条件下以及在半工业超声条件下获得的提取物中的蛋白质含量,并对最终放大的下游提取物的化学组成(碳水化合物、总酚和脂质)和元素分布进行了表征。

结果

这项工作发现,最终产品中蛋白质的最大含量为 378.31g.kg 的提取物。化学特性表明,每 1kg 提取物的平均含量为 326.19g 没食子酸当量的总酚,162.57g 葡萄糖当量的碳水化合物,以及 382.76g 亲脂性化合物。此外,当检查提取物中是否存在有害元素时,没有发现任何元素的含量可被认为对人类健康构成风险。

结论

所提出的半工业策略有可能通过制备富含蛋白质的提取物来极大地促进葡萄种子的增值,该提取物可用作合成葡萄酒稳定剂的替代品,并可用于开发新型食品和营养保健品。© 2024 化学工业协会。

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