Beijing International Scientific and Technological Cooperation Base of Water Pollution Control Techniques for Antibiotics and Resistance Genes, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard, School of Environment, Beijing Jiaotong University, Beijing 100044, China; Intelligent Environment Research Center, Beijing Jiaotong University, Beijing 100080, China.
Key Laboratory of Energy-Water Conservation and Wastewater Resources Recovery of China National Light Industry, Institute of Resources and Environment, Beijing Academy of Science and Technology, Beijing 100095, China.
Bioresour Technol. 2024 Apr;397:130463. doi: 10.1016/j.biortech.2024.130463. Epub 2024 Feb 17.
In this study, matrix degradation, microbial community development, and distribution using an individual-based model during biofilm formation on carriers at varying depths within a single-stage partial nitrification/anammox system were simulated. The findings from the application of individual-based model fitting, fluorescence in situ hybridization, and high-throughput sequencing reveal the presence of aerobic bacteria, specifically ammonia-oxidizing bacteria, as discrete particles within the outer layer of the carrier. Facultative anaerobic bacteria exemplified by anaerobic ammonia-oxidizing bacteria, are observed as aggregates within the middle layer. Conversely, anaerobic bacteria, represented by denitrifiers, are enveloped by extracellular polymeric substances within the inner layer. The present study extends the application of individual-based model to the formation of polyurethane-supported biofilms and presents valuable avenues for the design and advancement of pragmatic engineering carriers.
在这项研究中，使用个体模型模拟了在单级部分硝化/厌氧氨氧化系统中载体内部不同深度上生物膜形成过程中的基质降解、微生物群落发展和分布情况。个体模型拟合、荧光原位杂交和高通量测序的应用结果表明，有氧细菌，特别是氨氧化菌，作为载体外层的离散颗粒存在。兼性厌氧细菌，如厌氧氨氧化菌，被观察到在中层形成聚集体。相反，代表反硝化菌的厌氧细菌被包裹在细胞外聚合物物质的内层中。本研究将个体模型的应用扩展到了聚氨酯载体生物膜的形成，并为实用工程载体的设计和改进提供了有价值的途径。
