• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

评估 TaqMan Jr 基因分型法快速预测抗-Jr 抗体存在的方法。

Evaluating the TaqMan Jr-Genotyping Method for Rapidly Predicting the Presence of Anti-Jr Antibodies.

机构信息

Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.

出版信息

Ann Lab Med. 2024 Sep 1;44(5):418-425. doi: 10.3343/alm.2023.0325. Epub 2024 Feb 20.

DOI:10.3343/alm.2023.0325
PMID:38373791
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11169768/
Abstract

BACKGROUND

The Jr antigen is a high-prevalence red blood cell (RBC) antigen. Reports on cases of fatal hemolytic disease of the fetus and newborn and acute hemolytic transfusion reactions suggest that antibodies against Jr (anti-Jr) have potential clinical significance. Identifying anti-Jr is challenging owing to a lack of commercially available antisera. We developed an alternative approach to rapidly predict the presence of anti-Jr using the TaqMan single-nucleotide polymorphism (SNP)-genotyping method.

METHODS

Residual peripheral blood samples from 10 patients suspected of having the anti-Jr were collected. Two samples with confirmed Jr(a-) RBCs and anti-Jr were used to validate the TaqMan genotyping assay by comparing the genotyping results with direct sequencing. The accuracy of the assay in predicting the presence of anti-Jr was verified through crossmatching with in-house Jr(a-) O+ RBCs.

RESULTS

The TaqMan-genotyping method was validated with two Jr(a-) RBC- and anti-Jr-confirmed samples that showed concordant Jr genotyping and direct sequencing results. Jr genotyping for the remaining samples and crossmatching the serum samples with inhouse Jr(a-) O+ RBCs showed consistent results.

CONCLUSIONS

We validated a rapid, simple, accurate, and cost-effective method for predicting the presence of anti-Jr using a TaqMan-based SNP-genotyping assay. Implementing this method in routine practice in clinical laboratories will assist in solving difficult problems regarding alloantibodies to high-prevalence RBC antigens and ultimately aid in providing safe and timely transfusions and proper patient care.

摘要

背景

Jr 抗原是一种高频率出现的红细胞（RBC）抗原。有关胎儿和新生儿溶血病和急性溶血性输血反应的报告表明，针对 Jr 的抗体（抗-Jr）具有潜在的临床意义。由于缺乏商业上可获得的抗血清，因此鉴定抗-Jr 具有挑战性。我们开发了一种替代方法，使用 TaqMan 单核苷酸多态性（SNP）-基因分型方法来快速预测抗-Jr 的存在。

方法

收集了 10 例疑似存在抗-Jr 的患者的残留外周血样本。使用两种经确认 Jr(a-) RBC 和抗-Jr 的样本，通过将基因分型结果与直接测序进行比较，验证 TaqMan 基因分型检测的准确性。通过与内部 Jr(a-) O+ RBC 进行交叉配血，验证了该检测方法预测抗-Jr 存在的准确性。

结果

TaqMan 基因分型方法通过两种经确认 Jr(a-) RBC 和抗-Jr 的样本进行了验证，这两种样本的 Jr 基因分型和直接测序结果一致。对其余样本进行 Jr 基因分型，并将血清样本与内部 Jr(a-) O+ RBC 进行交叉配血，结果一致。

结论

我们验证了一种使用基于 TaqMan 的 SNP 基因分型检测快速、简单、准确且具有成本效益的方法来预测抗-Jr 的存在。在临床实验室常规实践中实施这种方法将有助于解决针对高频率 RBC 抗原的同种抗体的难题，并最终有助于提供安全及时的输血和适当的患者护理。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/ca27dfcfa451/alm-44-5-418-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/ae0f0bb338e5/alm-44-5-418-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/e81ef7c485f6/alm-44-5-418-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/ca27dfcfa451/alm-44-5-418-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/ae0f0bb338e5/alm-44-5-418-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/e81ef7c485f6/alm-44-5-418-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c146/11169768/ca27dfcfa451/alm-44-5-418-f3.jpg

相似文献

1
Evaluating the TaqMan Jr-Genotyping Method for Rapidly Predicting the Presence of Anti-Jr Antibodies.评估 TaqMan Jr 基因分型法快速预测抗-Jr 抗体存在的方法。
Ann Lab Med. 2024 Sep 1;44(5):418-425. doi: 10.3343/alm.2023.0325. Epub 2024 Feb 20.
2
When and why is red blood cell genotyping applicable in transfusion medicine: a systematic review of the literature.何时以及为何红细胞基因分型适用于输血医学：文献系统评价。
Immunohematology. 2024 Jun 24;40(2):58-64. doi: 10.2478/immunohematology-2024-009. eCollection 2024 Jun 1.
3
A Japanese multi-institutional collaborative study of antigen-positive red blood cell (RBC) transfusions in patients with corresponding RBC antibodies.日本的一项多机构合作研究，研究了具有相应 RBC 抗体的患者中抗原阳性 RBC 输血的情况。
Vox Sang. 2020 Jul;115(5):456-465. doi: 10.1111/vox.12906. Epub 2020 Mar 2.
4
High-throughput red blood cell antigen genotyping using a nanofluidic real-time polymerase chain reaction platform.基于纳流控实时聚合酶链反应平台的高通量红细胞抗原基因分型。
Transfusion. 2010 Jan;50(1):40-6. doi: 10.1111/j.1537-2995.2009.02377.x. Epub 2009 Sep 16.
5
Blood group antigen and phenotype prevalence in the Korean population compared to other ethnic populations and its association with RBC alloantibody frequency.与其他种族人群相比，韩国人群中血型抗原和表型的流行情况及其与红细胞同种抗体频率的关联。
Transfus Med. 2019 Dec;29(6):415-422. doi: 10.1111/tme.12643. Epub 2019 Oct 24.
6
HEA BeadChip™ technology in immunohematology.免疫血液学中的HEA BeadChip™技术。
Immunohematology. 2015;31(2):81-90.
7
Assessment of common red blood cell pretreatments to yield an accurate serologic antigen phenotype compared with genotype-predicted phenotype.评估常见的红细胞预处理方法，以获得与基因型预测表型相比准确的血清学抗原表型。
Immunohematology. 2019;33(4):147-151. doi: 10.21307/immunohematology-2019-020.
8
Performance of an automated solid-phase red cell adherence system compared with that of a manual gel microcolumn assay for the identification of antibodies eluted from red blood cells.自动固相红细胞黏附系统与手工凝胶微柱分析法在鉴定从红细胞洗脱的抗体方面的性能比较。
Immunohematology. 2011;27(1):1-5.
9
Anti-KANNO: a novel alloantibody against a red cell antigen of high frequency.抗-KANNO：一种针对高频红细胞抗原的新型同种抗体。
Transfus Med Rev. 2014 Jan;28(1):23-8. doi: 10.1016/j.tmrv.2013.12.001. Epub 2013 Dec 16.
10
Recombinant blood group proteins facilitate the detection of alloantibodies to high-prevalence antigens and reveal underlying antibodies: results of an international study.重组血型蛋白有助于检测针对高频率抗原的同种抗体并揭示潜在抗体：一项国际研究的结果。
Transfusion. 2014 Jul;54(7):1823-30. doi: 10.1111/trf.12553. Epub 2014 Mar 18.

本文引用的文献

1
The mutational constraint spectrum quantified from variation in 141,456 humans.从 141456 名人类个体的变异中量化的突变约束谱。
Nature. 2020 May;581(7809):434-443. doi: 10.1038/s41586-020-2308-7. Epub 2020 May 27.
2
KRGDB: the large-scale variant database of 1722 Koreans based on whole genome sequencing.KRGDB：基于全基因组测序的1722名韩国人的大规模变异数据库。
Database (Oxford). 2020 Jan 1;2020. doi: 10.1093/database/baaa030.
3
Fatal hemolytic disease of the fetus and newborn caused by anti-Jr antibody: A case report and literature review.
抗-Jr抗体所致胎儿及新生儿致命性溶血病：一例报告及文献复习
Transfus Apher Sci. 2020 Feb;59(1):102605. doi: 10.1016/j.transci.2019.06.029. Epub 2019 Jul 10.
4
Molecular analysis of immunized Jr(a-) or Lan- patients and validation of a high-throughput genotyping assay to screen blood donors for Jr(a-) and Lan- phenotypes.免疫的Jr(a-)或Lan-患者的分子分析以及用于筛选献血者Jr(a-)和Lan-血型表型的高通量基因分型检测方法的验证。
Transfusion. 2014 Jul;54(7):1836-46. doi: 10.1111/trf.12544. Epub 2014 Jan 24.
5
A review of the JR blood group system.JR血型系统综述。
Immunohematology. 2013;29(2):63-8.
6
Sensitization to multiple rh antigens by transfusion of random donor platelet concentrates in a -D- phenotype patient.在 D-表型患者中，输注随机供者血小板浓缩物导致对多种 Rh 抗原致敏。
Ann Lab Med. 2012 Nov;32(6):429-32. doi: 10.3343/alm.2012.32.6.429. Epub 2012 Oct 17.
7
The JR blood group system (ISBT 032): molecular characterization of three new null alleles.JR 血型系统（ISBT 032）：三个新的 null 等位基因的分子特征。
Transfusion. 2013 Jul;53(7):1575-9. doi: 10.1111/j.1537-2995.2012.03930.x. Epub 2012 Oct 15.
8
ABCG2 null alleles define the Jr(a-) blood group phenotype.ABCG2 无功能等位基因定义了 Jr(a-)血型表型。
Nat Genet. 2012 Jan 15;44(2):131-2. doi: 10.1038/ng.1075.
9
Null alleles of ABCG2 encoding the breast cancer resistance protein define the new blood group system Junior.ABCG2 编码乳腺癌耐药蛋白的无效等位基因定义了新的血液分组系统 Junior。
Nat Genet. 2012 Jan 15;44(2):174-7. doi: 10.1038/ng.1070.
10
Fatal hemolytic disease of the fetus and newborn possibly due to anti-Jr.
Transfusion. 2009 Apr;49(4):813. doi: 10.1111/j.1537-2995.2008.02087.x.