Jeong Dayi, Jang Goo, Jung Woo Kyung, Park Yong Ho, Bae Hojae
Department of Stem Cell and Regenerative Biotechnology, KU Convergence Science and Technology Institute, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
Laboratory of Theriogenology and Biotechnology, Department of Veterinary Clinical Science, College of Medicine and the Research Institute of Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
NPJ Sci Food. 2024 Feb 19;8(1):13. doi: 10.1038/s41538-024-00257-y.
Numerous studies have explored the cultivation of muscle cells using non-animal materials for cultivated meat production. Achieving muscle cell proliferation and alignment using 3D scaffolds made from plant-based materials remains challenging. This study introduces a technique to culture and align muscle cells using only plant-based materials, avoiding toxic chemical modifications. Zein-alginate fibers (ZA fibers) were fabricated by coating zein protein onto alginate fibers (A fibers). Zein's excellent cell compatibility and biodegradability enable high cell adhesion and proliferation rates, and the good ductility of the ZA fibers enable a high strain rate (>75%). We demonstrate mature and aligned myotube formation in ZA fibers, providing a simple way to align muscle cells using plant-based materials. Additionally, cultivated meat was constructed by assembling muscle, fat, and vessel fibers. This method holds promise for the future mass production of cultivated meat.
许多研究探索了使用非动物材料培养肌肉细胞用于生产人造肉。利用植物基材料制成的3D支架实现肌肉细胞增殖和排列仍然具有挑战性。本研究介绍了一种仅使用植物基材料培养和排列肌肉细胞的技术，避免了有毒的化学修饰。通过将玉米醇溶蛋白涂覆在海藻酸盐纤维（A纤维）上制备了玉米醇溶蛋白-海藻酸盐纤维（ZA纤维）。玉米醇溶蛋白优异的细胞相容性和生物降解性使得细胞具有高粘附率和增殖率，而ZA纤维良好的延展性使得应变率较高（>75%）。我们证明了在ZA纤维中形成了成熟且排列整齐的肌管，提供了一种使用植物基材料排列肌肉细胞的简单方法。此外，通过组装肌肉、脂肪和血管纤维构建了人造肉。这种方法有望在未来大规模生产人造肉。
