Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.
Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, MA, USA.
Commun Biol. 2022 Jun 2;5(1):466. doi: 10.1038/s42003-022-03423-8.
Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. However, research and development has been hindered by the lack of serum-free media that enable the robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described for pluripotent stem cells. Here, B8 is adapted for bovine satellite cells through the addition of a single component, recombinant albumin, which renders it suitable for long-term satellite cell expansion without sacrificing myogenicity. This new media (Beefy-9) maintains cell growth over the entire period tested (seven passages), with an average doubling time of 39 h. Along with demonstrated efficacy for bovine cells, Beefy-9 offers a promising starting-point for developing serum-free media for other meat-relevant species. Ultimately, this work offers a foundation for escaping cultured meat research's reliance on serum, thereby accelerating the field.
细胞培养肉具有更可持续、更合乎道德、更有弹性和更健康的食品系统的潜力。然而，由于缺乏无血清培养基，相关细胞（如肌肉卫星细胞）无法在多个传代中大量扩增，研究和开发受到了阻碍。最近，一种低成本的无血清培养基（B8）被描述用于多能干细胞。在这里，通过添加一种单一成分，重组白蛋白，将 B8 适应于牛卫星细胞，使其适合长期卫星细胞扩增，而不牺牲成肌能力。这种新的培养基（Beefy-9）在整个测试期间（七代）都能维持细胞生长，平均倍增时间为 39 小时。除了对牛细胞的有效性得到证实外，Beefy-9 还为开发其他与肉类相关的物种的无血清培养基提供了一个有希望的起点。最终，这项工作为摆脱细胞培养肉研究对血清的依赖奠定了基础，从而加速了该领域的发展。
