Monguillot Joaquin H, Arias Renee S, Orner Valerie A, Massa Alicia N, Sobolev Victor S, Lima Nelson Bernardi, Paredes Juan, Oddino Claudio, Carmona Marcelo, Conforto Cinthia
Instituto de Patologia Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, IPAVE-CIAP-INTA, Av. 11 de Septiembre, Córdoba 4755, Argentina.
Unidad de Fitopatologia y Modelizacion Agricola, Consejo Nacional de Investigaciones Científcas y Técnicas, UFyMA-CONICET, Av 11 de Septiembre, Córdoba 4755, Argentina.
Data Brief. 2024 Feb 6;53:110158. doi: 10.1016/j.dib.2024.110158. eCollection 2024 Apr.
Late leaf spot (LLS) caused by the Ascomycete (N.p.) (Syn. ) is the main foliar disease of peanuts in Argentina and in peanut producing areas of the world, causing up to 70% yield losses. The extremely slow growth of this fungus in culture, that takes around one month to form a 1 cm colony (0.45 mm/day), and the lack of adequate young tissues from where to extract nucleic acids, have hindered genetic studies of this pathogen. Here, we report the first genome sequence of a isolate from South America, as well as genetic variants on its conserved genes, and the complete sequence of its mating-type locus MAT1-2 idiomorph. The isolate IPAVE 0302 was obtained from peanut leaves in Córdoba, Argentina. The whole genome sequencing of IPAVE 0302 was performed as paired end 150 bp NovaSeq 6000 and assembled. Clean reads were mapped to the reference genome for this species NRRL 64463 and the genetic variants on highly conserved genes and throughout the genome were analyzed. Sequencing data were submitted to NCBI GenBank Bioproject PRJNA948451, accession number SRR23957761. Additional Fasta files are available from Harvard Dataverse (https://doi.org/10.7910/DVN/9AGPMG and https://doi.org/10.7910/DVN/YDO3V6). The data reported here will be the basis for the analysis of genetic diversity of the LLS pathogen of peanut in Argentina, information that is critical to make decisions on management strategies.
由子囊菌（N.p.）（同义词）引起的晚叶斑病（LLS）是阿根廷以及世界花生种植区花生的主要叶部病害，可导致高达70%的产量损失。这种真菌在培养基中生长极其缓慢，形成一个1厘米的菌落大约需要一个月时间（0.45毫米/天），而且缺乏用于提取核酸的合适幼嫩组织，这阻碍了对该病原体的遗传学研究。在此，我们报告了一株来自南美洲的分离株的首个基因组序列，以及其保守基因上的遗传变异，还有其交配型位点MAT1-2特异形态的完整序列。分离株IPAVE 0302从阿根廷科尔多瓦的花生叶片中获得。对IPAVE 0302进行了全基因组测序，采用150碱基对的双端NovaSeq 6000测序并进行组装。将过滤后的 reads 比对到该物种的参考基因组NRRL 64463上，并分析高度保守基因和整个基因组上的遗传变异。测序数据已提交至NCBI GenBank生物项目PRJNA948451，登录号为SRR23957761。其他Fasta文件可从哈佛数据文库获取（https://doi.org/10.7910/DVN/9AGPMG和https://doi.org/10.7910/DVN/YDO3V6）。这里报告的数据将作为分析阿根廷花生晚叶斑病病原体遗传多样性的基础，这些信息对于制定管理策略的决策至关重要。
