Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA.
J Magn Reson Imaging. 2024 Nov;60(5):2196-2204. doi: 10.1002/jmri.29291. Epub 2024 Feb 20.
Recently, dynamic contrast-enhanced (DCE) MRI with ferumoxytol as contrast agent has recently been introduced for the noninvasive assessment of placental structure and function throughout. However, it has not been demonstrated under pathological conditions.
To measure cotyledon-specific rhesus macaque maternal placental blood flow using ferumoxytol DCE MRI in a novel animal model for local placental injury.
Prospective animal model.
Placental injections of Tisseel (three with 0.5 mL and two with 1.5 mL), monocyte chemoattractant protein 1 (three with 100 μg), and three with saline as controls were performed in a total of 11 rhesus macaque pregnancies at approximate gestational day (GD 101). DCE MRI scans were performed prior (GD 100) and after (GD 115 and GD 145) the injection (term = GD 165).
FIELD STRENGTH/SEQUENCE: 3 T, T1-weighted spoiled gradient echo sequence (product sequence, DISCO).
Source images were inspected for motion artefacts from the mother or fetus. Placenta segmentation and DCE processing were performed for the dynamic image series to measure cotyledon specific volume, flow, and normalized flow. Overall placental histopathology was conducted for controls, Tisseel, and MCP-1 animals and regions of tissue infarctions and necrosis were documented. Visual inspections for potential necrotic tissue were conducted for the two Tisseelx3 animals.
Wilcoxon rank sum test, significance level P < 0.05.
No motion artefacts were observed. For the group treated with 1.5 mL of Tisseel, significantly lower cotyledon volume, flow, and normalized flow per cotyledon were observed for the third gestational time point of imaging (day ~145), with mean normalized flow of 0.53 minute. Preliminary histopathological analysis shows areas of tissue necrosis from a selected cotyledon in one Tisseel-treated (single dose) animal and both Tisseelx3 (triple dose) animals.
This study demonstrates the feasibility of cotyledon-specific functional analysis at multiple gestational time points and injury detection in a placental rhesus macaque model through ferumoxytol-enhanced DCE MRI.
NA TECHNICAL EFFICACY: Stage 2.
最近，使用 Ferumoxytol 作为对比剂的动态对比增强（DCE）MRI 已被引入用于无创评估胎盘结构和功能。然而，它在病理条件下尚未得到证实。
在局部胎盘损伤的新型动物模型中，使用 Ferumoxytol DCE MRI 测量恒河猴胎盘绒毛叶特异性母体胎盘血流。
前瞻性动物模型。
在总共 11 例恒河猴妊娠中，胎盘注射 Tisseel（3 例注射 0.5ml，2 例注射 1.5ml）、单核细胞趋化蛋白 1（3 例注射 100μg）和生理盐水作为对照，在注射前（GD100）和注射后（GD115 和 GD145）进行 DCE MRI 扫描（足月=GD165）。
磁场强度/序列：3T，T1 加权扰相梯度回波序列（产品序列，DISCO）。
检查来自母亲或胎儿的运动伪影的源图像。对动态图像系列进行胎盘分割和 DCE 处理，以测量绒毛叶特异性体积、流量和归一化流量。对 Tisseel 和 MCP-1 动物的所有胎盘进行组织病理学检查，并记录组织梗死和坏死区域。对 2 例 Tisseelx3 动物进行了潜在坏死组织的视觉检查。
Wilcoxon 秩和检验，显著性水平 P<0.05。
未观察到运动伪影。对于接受 1.5ml Tisseel 治疗的组，在成像的第三个妊娠时间点（约 145 天），观察到绒毛叶体积、流量和每个绒毛叶的归一化流量显著降低，平均归一化流量为 0.53 分钟。初步组织病理学分析显示，来自接受 Tisseel 治疗（单次剂量）的一只动物和接受 Tisseelx3（三次剂量）的两只动物的一个选定绒毛叶的组织坏死区域。
本研究通过 Ferumoxytol 增强 DCE MRI，证明了在胎盘恒河猴模型中进行多时间点绒毛叶特异性功能分析和损伤检测的可行性。
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2 级。
