多菌灵在Muta小鼠肝脏和腺胃中无体内致突变性。
Lack of in vivo mutagenicity of carbendazim in the liver and glandular stomach of MutaMice.
作者信息
Iso Takako, Suzuki Kenichiro, Murata Yasumasa, Hirose Nozomu, Umano Takaaki, Horibata Katsuyoshi, Sugiyama Kei-Ichi, Hirose Akihiko, Masumura Kenichi, Matsumoto Mariko
机构信息
Division of Risk Assessment, National Institute of Health Sciences, Kanagawa, Japan.
Genotoxicology Laboratory, BioSafety Research Center Inc., Shizuoka, Japan.
出版信息
Genes Environ. 2024 Feb 20;46(1):7. doi: 10.1186/s41021-024-00299-4.
BACKGROUND
Carbendazim (methyl 2-benzimidazolecarbamate, CASRN: 10605-21-7) exhibits spindle poisoning effects and is widely used as a fungicide. With respect to genotoxicity, carbendazim is deemed to be non-mutagenic in vitro, but it causes indicative DNA damage in vivo and chromosome aberrations in vitro and in vivo. In this study, we examined the mutagenicity of carbendazim in vivo.
RESULTS
MutaMice were treated with carbendazim orally at doses of 0 (corn oil), 250, 500, and 1,000 mg/kg/day once a day for 28 days. A lacZ assay was used to determine the mutant frequency (MF) in the liver and glandular stomach of mice. MutaMice were administered up to the maximum dose recommended by the Organization for Economic Co-operation and Development Test Guidelines for Chemicals No. 488 (OECD TG488). The lacZ MFs in the liver and glandular stomach of carbendazim-treated animals were not significantly different from those in the negative control animals. In contrast, positive control animals exhibited a significant increase in MFs in both the liver and glandular stomach.
CONCLUSIONS
Carbendazim is non-mutagenic in the liver and glandular stomach of MutaMice following oral treatment.
背景
多菌灵（2-苯并咪唑氨基甲酸甲酯，化学物质登记号：10605-21-7）具有纺锤体毒害作用，被广泛用作杀菌剂。关于遗传毒性，多菌灵在体外被认为无致突变性，但在体内会导致指示性DNA损伤，在体外和体内均可引起染色体畸变。在本研究中，我们检测了多菌灵在体内的致突变性。
结果
用多菌灵以0（玉米油）、250、500和1000毫克/千克/天的剂量对MutaMice小鼠进行口服处理，每天一次，持续28天。采用lacZ分析法测定小鼠肝脏和腺胃中的突变频率（MF）。给予MutaMice小鼠的剂量高达经济合作与发展组织化学品测试指南第488号（OECD TG488）推荐的最大剂量。多菌灵处理动物的肝脏和腺胃中的lacZ突变频率与阴性对照动物相比无显著差异。相反，阳性对照动物的肝脏和腺胃中的突变频率均显著增加。
结论
口服处理后，多菌灵在MutaMice小鼠的肝脏和腺胃中无致突变性。
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