Department of Anatomy, Ziauddin University.
Department of Molecular Medicine, Ziauddin University.
J Pak Med Assoc. 2024 Feb;74(1 (Supple-2)):S51-S58. doi: 10.47391/JPMA-DUHS-S11.
To isolate a homogenous population of human amniotic epithelial cells (hAECs) from the amniotic membrane of the human placenta and differentiate them into hepatic-like cells with the help of small molecules.
hAECs were isolated by using the enzymatic digestion method and characterized for the presence of specific stem cell markers. In-vitro, hepatic differentiation of hAECs was carried out by using a combination of small molecules. Differentiated cells were observed under a live cell imaging microscope for morphological changes followed by gene and protein expression analysis by qPCR and immunocytochemistry respectively.
The isolated hAECs attained characteristic cuboid epithelial shape and express stem cells marker. The hepatic differentiation method was optimized based on soluble chemical compounds supplied in the culture medium. The differentiated hAECs phenotypically acquire hepatic-like cell features and expressed hepatic markers as well as hepatic protein albumin at immature levels.
The isolated population of hAECs is highly proliferative. Moreover, hepatic markers expression in the isolated hAECs makes them an exclusive source for the treatment of chronic liver diseases.
从人胎盘羊膜中分离出具有均一表型的人羊膜上皮细胞（hAEC），并借助小分子将其诱导分化为肝样细胞。
采用酶消化法分离 hAEC，并通过特异性干细胞标志物进行鉴定。体外实验中，通过小分子组合诱导 hAEC 向肝系细胞分化。在活细胞成像显微镜下观察细胞形态变化，随后通过 qPCR 和免疫细胞化学法分别进行基因和蛋白表达分析。
分离得到的 hAEC 呈典型的立方上皮样形态，表达干细胞标志物。基于培养基中提供的可溶性化学化合物，优化了肝向分化方法。分化后的 hAEC 表型上获得肝样细胞特征，并表达肝标志物以及不成熟水平的肝蛋白白蛋白。
分离得到的 hAEC 群体具有高增殖能力。此外，hAEC 中肝标志物的表达使其成为治疗慢性肝脏疾病的独特来源。
