U.S. Department of Agriculture-Agricultural Research Service-Tropical Agriculture Research Station (TARS), 2200 P.A. Campos Ave., Ste. 201, Mayagüez, PR 00680-5470.
Department of Biology, University of Puerto Rico-Río Piedras, P.O. Box 23360, San Juan, PR 00931-3360.
Phytopathology. 2024 Jun;114(6):1320-1332. doi: 10.1094/PHYTO-02-24-0046-R. Epub 2024 Jun 18.
Coffee fruit rot (CFR) is a well-known disease worldwide, mainly caused by spp., the most important species being subsp. . In Puerto Rico, spp. were identified as pathogens of coffee fruits. The coffee berry borer (CBB) was shown to be a dispersal agent of these fungi, and interaction of with affecting coffee fruits was suggested. In this study, we demonstrated that spp. also cause CFR in Puerto Rico. spp. are part of the CBB mycobiota, and this insect is responsible for spreading the pathogens in coffee fields. We identified nine spp. (, and ) belonging to six species complexes isolated from CBBs and from rotten coffee fruits. Pathogenicity tests showed that , and were pathogens causing CFR on green coffee fruits. was the predominant species isolated from the CBB mycobiota and coffee fruits with symptoms of CFR, suggesting a close relationship between and the CBB. To our knowledge, this is the first report of , and causing CFR worldwide and the first report of causing CFR in Puerto Rico. Understanding the CFR disease complex and how the CBB contributes to dispersing different spp. on coffee farms is important to implement disease management practices in Puerto Rico and in other coffee-producing countries.
咖啡浆果腐烂病（CFR）是一种在世界范围内广为人知的疾病，主要由 spp.引起，其中最重要的物种是 subsp. 。在波多黎各， spp.被鉴定为咖啡果实的病原体。咖啡果蛀虫（CBB）被证明是这些真菌的传播媒介，并且推测 与 之间的相互作用会影响咖啡果实。在这项研究中，我们证明 spp.也会导致波多黎各的 CFR。 spp.是 CBB 真菌群的一部分，这种昆虫负责在咖啡田中传播病原体。我们从 CBB 和腐烂的咖啡果实中分离出了九个 spp.（、和 ），它们属于六个 物种复合体。致病性测试表明 、和 是引起绿色咖啡果实 CFR 的病原体。从 CBB 真菌群和有 CFR 症状的咖啡果实中分离出的主要物种是 ，这表明 与 CBB 之间存在密切关系。据我们所知，这是首次在全球范围内报道 spp.、和 引起 CFR，也是首次在波多黎各报道 引起 CFR。了解 CFR 疾病复合体以及 CBB 如何在咖啡农场中传播不同的 spp.，对于在波多黎各和其他咖啡生产国实施疾病管理实践非常重要。
