1α,25-二羟基维生素D诱导的胚胎鸡肠道中一种胞质蛋白的修饰

1 alpha,25-Dihydroxyvitamin D-induced modification of a cytosolic protein in embryonic chick intestine.

作者信息

Bishop C W, Kendrick N C, Dame M C, DeLuca H F

出版信息

J Biol Chem. 1985 May 10;260(9):5209-12.

Abstract

Two-dimensional electrophoresis together with radiolabeling experiments was used to examine cytosolic proteins of embryonic chick duodenum for responses to 1,25-dihydroxyvitamin D3. 1,25-Dihydroxyvitamin D3 caused a striking decrease in [3H]leucine content of an 18,000-dalton protein (approximate pI, 5.1) after a 10-min pulse with radioisotope followed by a 4-h chase. Decreased [14C]leucine content of the same protein was also observed at various times following 1,25-dihydroxyvitamin D3 addition to culture media; a significant decrease in radiolabel incorporation occurred within 30 min after addition of the hormone. The results argue that 1,25-dihydroxyvitamin D3 causes either a decreased synthesis rate or a post-translational modification of this protein. This change joins the biosynthesis of calcium-binding protein as an early event in the response of chick embryonic intestine to 1,25-dihydroxyvitamin D3.

摘要

二维电泳结合放射性标记实验被用于检测胚胎期鸡十二指肠的胞质蛋白对1,25 - 二羟维生素D3的反应。在用放射性同位素脉冲标记10分钟后再追踪4小时，1,25 - 二羟维生素D3导致一种18000道尔顿蛋白质（近似等电点为5.1）的[3H]亮氨酸含量显著下降。在向培养基中添加1,25 - 二羟维生素D3后的不同时间，也观察到了该蛋白[14C]亮氨酸含量的下降；在添加激素后30分钟内，放射性标记掺入量就出现了显著下降。结果表明，1,25 - 二羟维生素D3导致该蛋白的合成速率降低或发生翻译后修饰。这种变化与钙结合蛋白的生物合成一起，成为鸡胚胎肠道对1,25 - 二羟维生素D3反应的早期事件。