正常和糖尿病大鼠主动脉对去内皮作用在6-酮-前列腺素F1α合成及胆固醇蓄积方面的反应。

Response of normal and diabetic rat aorta to deendothelialization in terms of 6-keto-prostaglandin F1 alpha synthesis and cholesterol accumulation.

作者信息

Shehata-Khalil M, Wey H E, Subbiah M T

出版信息

Biochem Med. 1985 Feb;33(1):22-8. doi: 10.1016/0006-2944(85)90122-x.

Abstract

The response of rat aorta to balloon catheter-induced denudation in terms of 6-keto-PGF1 alpha synthesis and cholesterol accumulation was studied in normal and streptozotocin-induced diabetic animals. Initial studies in control rats showed that 1 day after deendothelialization, aortic 6-keto-PGF1 alpha was significantly reduced (31%), and this was normalized at 4 days after injury. However, endothelial regeneration as indicated by Evans blue staining was complete only at 14 days after denudation. Diabetic aorta showed a more severe reduction of 6-keto-PGF1 alpha production (50%) at 1 day after deendothelialization, but this was also normalized at 4 days after injury. Deendothelialization did not increase the accumulation of cholesterol in diabetic aorta. It is concluded that (a) recovery of PGI2 production in deendothelialized aorta does not parallel endothelial regeneration, indicating substantial contribution of subendothelial layer, in rats and (b) short-term aortic injury in diabetic rat will not influence the arterial repair process and cholesterol accumulation and is unlikely to be a contributing factor to increased atherogenesis.

摘要

在正常和链脲佐菌素诱导的糖尿病动物中，研究了大鼠主动脉对球囊导管诱导的内皮剥脱在6-酮-前列环素F1α合成和胆固醇积累方面的反应。对对照大鼠的初步研究表明，内皮剥脱后1天，主动脉6-酮-前列环素F1α显著降低（31%），损伤后4天恢复正常。然而，伊文思蓝染色显示的内皮再生仅在剥脱后14天完成。糖尿病大鼠主动脉在内皮剥脱后1天6-酮-前列环素F1α产生的降低更为严重（50%），但损伤后4天也恢复正常。内皮剥脱并未增加糖尿病大鼠主动脉中胆固醇的积累。结论是：（a）内皮剥脱的主动脉中前列环素I2产生的恢复与内皮再生不同步，表明大鼠内皮下层有重要作用；（b）糖尿病大鼠的短期主动脉损伤不会影响动脉修复过程和胆固醇积累，不太可能是动脉粥样硬化增加的一个促成因素。