Jacobs H C, Jobe A H, Ikegami M, Jones S
Biochim Biophys Acta. 1985 Apr 25;834(2):172-9. doi: 10.1016/0005-2760(85)90153-5.
Developing rabbits reutilize the phosphatidylcholine of surfactant with an efficiency of about 95%. The efficiency of reutilization of other components of surfactant have not been determined. 3-day-old rabbits were injected intratracheally with [3H]dipalmitoylphosphatidylcholine (DPPC) mixed with unlabeled natural surfactant and either disaturated [32P]phosphatidylglycerol (DSPG) or [14C]dipalmitoylphosphatidyl-ethanolamine (DPPE). The recovery of [3H]DPPC, [14C]DPPE, and [32P]DSPG in the alveolar wash was measured at different times after injection. By plotting the ratio of [32P]DSPG to [3H]DPPC or [14C]DPPE to [3H]DPPC counts/min in the alveolar wash vs. time after injection we showed that these two phospholipids are reutilized less efficiently than phosphatidylcholine. Based on other studies, several assumptions were made about the kinetics of surfactant phosphatidylethanolamine and phosphatidylglycerol. From the slopes of the semilog plots of total [14C]DPPE and total [32P]DSPG counts/min in the alveolar wash vs. time and these assumptions, we determined that these two phospholipids were reutilized at an efficiency of only 79%.
发育中的兔子对表面活性剂中磷脂酰胆碱的再利用效率约为95%。表面活性剂其他成分的再利用效率尚未确定。给3日龄的兔子经气管内注射与未标记的天然表面活性剂混合的[3H]二棕榈酰磷脂酰胆碱（DPPC），以及二饱和[32P]磷脂酰甘油（DSPG）或[14C]二棕榈酰磷脂酰乙醇胺（DPPE）。在注射后的不同时间测量肺泡灌洗中[3H]DPPC、[14C]DPPE和[32P]DSPG的回收率。通过绘制肺泡灌洗中[32P]DSPG与[3H]DPPC的比值或[14C]DPPE与[3H]DPPC的计数/分钟与注射后时间的关系图，我们发现这两种磷脂的再利用效率低于磷脂酰胆碱。基于其他研究，对表面活性剂磷脂酰乙醇胺和磷脂酰甘油的动力学做出了一些假设。根据肺泡灌洗中总[14C]DPPE和总[32P]DSPG计数/分钟与时间的半对数图的斜率以及这些假设，我们确定这两种磷脂的再利用效率仅为79%。
