Jacobs H C, Ikegami M, Jobe A H, Berry D D, Jones S
Biochim Biophys Acta. 1985 Oct 23;837(1):77-84. doi: 10.1016/0005-2760(85)90087-6.
32P-saturated phosphatidylcholine was added to [3H]choline-labeled natural surfactant and the mixture was injected intratracheally into 87 adult rabbits. The rabbits were also given [14C]palmitate intravenously at the same time. Rabbits were killed in groups from 10 min to 72 h after injection. In each rabbit we measured the total recovered [3H]phosphatidylcholine (PC) in the alveolar wash, the ratio of [3H]PC to [32P]PC in the alveolar wash, and the specific activity of [14C]PC in the alveolar wash and lamellar bodies. Values were averaged for all rabbits killed at the same times and smooth curves were fit to the data by computer. From the intravenous [14C]palmitate data we calculated a turnover time for alveolar PC of 6.0 h. From the intratracheal labeling data, we calculated a turnover time for alveolar PC of 5.7 h and determined that alveolar PC was reutilized at an efficiency of only 23%. We also concluded that this reutilization occurred as intact molecules.
将32P饱和磷脂酰胆碱添加到[3H]胆碱标记的天然表面活性剂中，并将混合物经气管内注入87只成年兔体内。同时，这些兔子还静脉注射了[14C]棕榈酸酯。在注射后10分钟至72小时内，将兔子分组处死。在每只兔子中，我们测量了肺泡灌洗中回收的总[3H]磷脂酰胆碱（PC）、肺泡灌洗中[3H]PC与[32P]PC的比例，以及肺泡灌洗和板层小体中[14C]PC的比活性。对在同一时间处死的所有兔子的数据进行平均，并通过计算机对数据拟合出平滑曲线。根据静脉注射[14C]棕榈酸酯的数据，我们计算出肺泡PC的周转时间为6.0小时。根据气管内标记数据，我们计算出肺泡PC的周转时间为5.7小时，并确定肺泡PC的再利用效率仅为23%。我们还得出结论，这种再利用是以完整分子的形式发生的。
