Wu Xiying, Chen Hao-Wei, Zhao Ze-Yu, Li Lisha, Song Chi, Xiong Juan, Yang Guo-Xun, Zhu Quangang, Hu Jin-Feng
School of Pharmaceutical Sciences, Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University Zhejiang 318000 China
Department of Natural Medicine, School of Pharmacy, Fudan University Shanghai 201203 China
RSC Med Chem. 2024 Jan 3;15(2):553-560. doi: 10.1039/d3md00611e. eCollection 2024 Feb 21.
With the increasing prevalence of infections, rapid emergence of drug resistance and the slow healing of infected wounds, developing an efficient antibiotic-free multifunctional wound dressing for inhibiting and simultaneously facilitating wound healing have become a huge challenge. Due to their excellent biocompatibility and biodegradability, some carbopol hydrogels based on plant extracts or purified compounds have already been applied in wound healing treatment. In China, Willd. (EuH) has been traditionally used as a medicine and food homologous medicine for the treatment of furuncles and carbuncles mainly caused by infection. In an earlier study, EuH-originated flavonoids quercetin (QU) and luteolin (LU) could serve as a potential source for anti- drug discovery when used in synergy. However, the effects of QU and LU on -infected wound healing are still unknown. In this study, we found a series of Carbopol 940-based hydrogels loading QU and LU in combination could disinfect and also could promote wound healing. In the full-thickness skin defect mouse model infected with , the wound contraction ratio, bacterial burden, skin hyperplasia and inflammation score, as well as collagen deposition and blood vessels were then investigated. The results indicate that the optimized QL2 [QU (32 μg mL)-LU (8 μg mL)] hydrogel with biocompatibility significantly promoted -infected wound healing through anti-infection, anti-inflammation, collagen deposition, and angiogenesis, revealing it as a promising alternative for infected wound repair.
随着感染发生率的不断上升、耐药性的迅速出现以及感染伤口愈合缓慢，开发一种高效的无抗生素多功能伤口敷料以抑制并同时促进伤口愈合已成为一项巨大挑战。由于其优异的生物相容性和生物降解性，一些基于植物提取物或纯化化合物的卡波姆水凝胶已应用于伤口愈合治疗。在中国，地锦（EuH）传统上被用作药食同源药物，主要用于治疗由金黄色葡萄球菌感染引起的疖肿。在早期研究中，来源于地锦的黄酮类化合物槲皮素（QU）和木犀草素（LU）协同使用时可作为抗耐药药物发现的潜在来源。然而，QU和LU对金黄色葡萄球菌感染伤口愈合的影响仍不清楚。在本研究中，我们发现一系列负载QU和LU的卡波姆940基水凝胶既能消毒又能促进伤口愈合。在感染金黄色葡萄球菌的全层皮肤缺损小鼠模型中，随后研究了伤口收缩率、细菌负荷、皮肤增生和炎症评分，以及胶原蛋白沉积和血管情况。结果表明，具有生物相容性的优化QL2 [QU（32μg/mL）-LU（8μg/mL）]水凝胶通过抗感染、抗炎、胶原蛋白沉积和血管生成显著促进了金黄色葡萄球菌感染伤口的愈合，表明它是感染伤口修复的一种有前景的替代物。
