Borbye-Lorenzen Nis, Deza-Lougovski Yacila I, Holmgaard Solveig, Weiss Luzia M, Bækvad-Hansen Marie, Skogstrand Kristin, Rieckmann Anna, Börsch-Supan Axel, Börsch-Supan Martina
Department of Congenital Disorders Center for Neonatal Screening, Statens Serum Institut Copenhagen Denmark.
Institute of Psychology University of the Bundeswehr München Neubiberg Germany.
Alzheimers Dement (Amst). 2024 Feb 22;16(1):e12555. doi: 10.1002/dad2.12555. eCollection 2024 Jan-Mar.
The apolipoprotein E () ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings.
We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE).
The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences.
Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.
载脂蛋白E（apoE）ε4等位基因与患阿尔茨海默病的高风险相关。目前尚不清楚ε4携带者中循环apoE4蛋白的个体水平是否会带来额外风险。从干血斑（DBS）中测量apoE4蛋白水平有可能提供有关遗传状态以及循环水平的信息，并将这些测量纳入大规模调查中。
我们开发了一种多重免疫测定法，以检测来自欧洲健康、老龄化和退休调查（SHARE）第6波的15974名50岁及以上参与者的DBS中的apoE4蛋白水平。
apoE4蛋白信号呈现出两种可分离的分布。一种分布对应于至少携带一个ε4等位基因拷贝的携带者。现场工作共同因素影响蛋白水平，但无法解释个体差异。
未来的研究应调查基因型和apoE4水平如何与生活方式及其他变量相互作用，以影响认知衰老。
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