Institute of Psychology, University of the Bundeswehr München, Neubiberg, Germany.
Max Planck Institute for Social Law and Social Policy, Munich, Germany.
Alzheimers Dement. 2024 Nov;20(11):7613-7623. doi: 10.1002/alz.14224. Epub 2024 Sep 5.
The apolipoprotein E (APOE) ε4 allele carries risk for cognitive impairment, but whether the level of circulating apoE4 protein in carriers affects cognition is unclear, as is how health and lifestyle impact circulating apoE4 levels.
We assayed apoE4 protein levels in dried blood spots of 12,532 adults aged 50+. Regression analyses tested the likelihood of cognitive impairment between groups and within those with detected apoE4 protein. Predictors of circulating apoE4 were assessed.
We detected protein binding that indicates the presence of an APOE ε4 allele in 28.4% of this group. This group was more likely to have cognitive impairment, and this risk increases with age. However, higher apoE4 levels were associated with less likelihood of cognitive impairment within this group. Antihypertensive medication predicted apoE4 protein levels.
The apoE4 isoform is associated with a deficient protein and worse cognition. This association is modulated by the level of circulating apoE4 protein in ε4 carriers.
An assay to quantify apoE4 levels from dried blood spot samples was applied. The apoE4 protein was detected as specific binding at ≥30,000 pg/mL in 28.4% of samples. Having the apoE4 protein was associated with worse cognitive performance. Higher apoE4 protein levels in those who have it were associated with better cognition. Cardiovascular factors influenced levels of apoE4 protein.
载脂蛋白 E (APOE) ε4 等位基因携带认知障碍风险，但携带者循环 APOE4 蛋白水平是否影响认知尚不清楚，健康和生活方式如何影响循环 APOE4 水平也不清楚。
我们检测了 12532 名 50 岁以上成年人的干血斑中的 APOE4 蛋白水平。回归分析测试了认知障碍在各组之间和在检测到 APOE4 蛋白的组内的可能性。评估了循环 APOE4 的预测因子。
我们在该组中检测到表明存在 APOE ε4 等位基因的蛋白结合，占 28.4%。该组更有可能出现认知障碍，且这种风险随年龄增长而增加。然而，在该组内，较高的 APOE4 水平与认知障碍的可能性降低相关。抗高血压药物预测 APOE4 蛋白水平。
APOE4 同工型与蛋白不足和认知障碍有关。这种关联在 ε4 携带者的循环 APOE4 蛋白水平上受到调节。
应用了一种从干血斑样本中定量 APOE4 水平的测定方法。在≥30000pg/mL 处检测到特异性结合的 APOE4 蛋白，在 28.4%的样本中检测到。存在 APOE4 蛋白与认知表现更差相关。在有 APOE4 蛋白的人群中，较高的 APOE4 蛋白水平与更好的认知相关。心血管因素影响 APOE4 蛋白水平。
