Forde B G, Heyworth A, Pywell J, Kreis M
Nucleic Acids Res. 1985 Oct 25;13(20):7327-39. doi: 10.1093/nar/13.20.7327.
The B-hordeins are the major group of prolamin storage proteins in barley (Hordeum vulgare L.) and they are encoded by a small multigene family that is expressed specifically in the developing endosperm. We report the complete nucleotide sequence of a clone of one B-hordein gene (pBHR184). The cloned gene contains no introns and belongs to the B1 sub-family of B-hordein genes. Comparison of the 5'-flanking sequences of pBHR184 with those of related S-rich prolamin genes from wheat shows that several short sequences within 600 bp upstream of the translation initiation codon are strongly conserved. A sequence that is conserved at around -300 bp in the S-rich prolamins is also conserved at similar locations in genes encoding the two major classes of maize prolamin (the Z19 and Z21 zeins) and appears to be unique to prolamin genes. We discuss the possible role of this '-300 element' in the control of gene expression in the developing cereal endosperm.
B族醇溶蛋白是大麦（Hordeum vulgare L.）中醇溶蛋白贮藏蛋白的主要类型，它们由一个小的多基因家族编码，该家族在发育中的胚乳中特异性表达。我们报道了一个B族醇溶蛋白基因（pBHR184）克隆的完整核苷酸序列。克隆的基因不含内含子，属于B族醇溶蛋白基因的B1亚家族。将pBHR184的5'侧翼序列与来自小麦的相关富含S的醇溶蛋白基因的序列进行比较，结果表明，翻译起始密码子上游600 bp内的几个短序列高度保守。在富含S的醇溶蛋白中，大约在-300 bp处保守的一个序列在编码玉米两种主要醇溶蛋白类型（Z19和Z21玉米醇溶蛋白）的基因的相似位置也保守，并且似乎是醇溶蛋白基因所特有的。我们讨论了这个“-300元件”在控制谷类发育胚乳中基因表达方面的可能作用。
