Mirani-Oostdijk C P, Havekes L, den Boer P J, de Wit E, Terpstra J
Scand J Clin Lab Invest. 1985 May;45(3):207-15. doi: 10.3109/00365518509160997.
Serum samples, obtained at various times of the day from four normal subjects and five patients with primary endogenous hypertriglyceridaemia on a carbohydrate-rich diet (meals at 09.00, 13.00 and 17.00 h), were incubated with cows' milk lipoprotein lipase (LPL) to investigate the susceptibility of the triglyceride(TG)-rich lipoprotein fraction to hydrolysis. Mean KM and vmax of this reaction increased in patients between 09.00 and 13.00 h (p = 0.016 and 0.015, respectively) and decreased again thereafter, whereas no diurnal change was observed in normal subjects (p = 0.31 and 0.27, respectively). At 13.00 and 23.00 h patients showed a higher mean KM (p less than 0.02) at 23.00 h a higher mean vmax (p less than 0.03) than normal subjects. When the data were expressed as reaction rates (FFA formation rates) at TG-concentrations in the in vivo range, neither group showed a diurnal difference. Patients showed a significantly lower reaction rate than normal subjects only at 13.00 h at TG concentrations below 3 mmol X 1-1. This suggests that a defect in the suitability of TG-rich particles to serve as a substrate for LPL is not likely to play a role in the development of hypertriglyceridaemia.
从四名正常受试者和五名原发性内源性高甘油三酯血症患者身上，于一天中的不同时间采集血清样本（这些患者采用富含碳水化合物的饮食，分别在09:00、13:00和17:00进食），将其与牛乳脂蛋白脂肪酶（LPL）一起孵育，以研究富含甘油三酯（TG）的脂蛋白组分对水解的敏感性。在患者中，该反应的平均米氏常数（KM）和最大反应速度（vmax）在09:00至13:00之间升高（p分别为0.016和0.015），此后再次下降，而正常受试者未观察到昼夜变化（p分别为0.31和0.27）。在13:00和23:00时，患者的平均KM高于正常受试者（p＜0.02），在23:00时平均vmax高于正常受试者（p＜0.03）。当数据表示为体内范围内TG浓度下的反应速率（游离脂肪酸形成速率）时，两组均未显示出昼夜差异。仅在TG浓度低于3 mmol·L⁻¹时，患者在13:00时的反应速率显著低于正常受试者。这表明富含TG的颗粒作为LPL底物的适宜性缺陷不太可能在高甘油三酯血症的发生中起作用。
