Laboratory of Nano-Photonics and Biosensors, Institute of Electro-Optical Engineering, National Taiwan Normal University, Taipei 11677, Taiwan.
Department of Life Science, National Taiwan Normal University, Taipei 11677, Taiwan.
Biosensors (Basel). 2024 Feb 13;14(2):99. doi: 10.3390/bios14020099.
Surface Plasmon Resonance (SPR) technology is known to be a powerful tool for studying biomolecular interactions because it offers real-time and label-free multiparameter analysis with high sensitivity. This article summarizes the results that have been obtained from the use of SPR technology in studying the dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations. This paper will begin by introducing the working principle of SPR and the kinetic parameters of the sensorgram, which include the association rate constant (k), dissociation rate constant (k), equilibrium association constant (K), and equilibrium dissociation constant (K). At the end of the paper, we will summarize the kinetic data on the interaction between angiotensin-converting enzyme 2 (ACE2) and SARS-CoV-2 obtained from the results of SPR signal analysis. ACE2 is a material that mediates virus entry. Therefore, understanding the kinetic changes between ACE2 and SARS-CoV-2 caused by the mutation will provide beneficial information for drug discovery, vaccine development, and other therapeutic purposes.
表面等离子体共振（SPR）技术是一种研究生物分子相互作用的强大工具，因为它提供了实时、无标记的多参数分析，具有高灵敏度。本文总结了使用 SPR 技术研究严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）突变动力学的结果。本文首先介绍 SPR 的工作原理和传感器图的动力学参数，包括缔合速率常数（k）、解离速率常数（k）、平衡缔合常数（K）和平衡解离常数（K）。最后，我们将总结从 SPR 信号分析结果中获得的血管紧张素转换酶 2（ACE2）与 SARS-CoV-2 相互作用的动力学数据。ACE2 是介导病毒进入的物质。因此，了解 ACE2 和 SARS-CoV-2 之间由于突变引起的动力学变化将为药物发现、疫苗开发和其他治疗目的提供有益信息。
