School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City 545006, Guangxi, China.
School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City 545006, Guangxi, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123275. doi: 10.1016/j.saa.2023.123275. Epub 2023 Aug 18.
The global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a great disaster to the economy and human society. Nowadays, SARS-CoV-2 is fading away from people's memory but it still exists around us. PCR plays an important role in detecting SARS-CoV-2 but it requires a long detecting time, equipped laboratory, and professional operators. In comparison with polymerase chain reaction (PCR), surface-enhanced Raman scattering (SERS) is a promising method for detecting SARS-CoV-2 due to its fast, easily operated, and high-sensitivity properties. In this study, the monolayer Ag nanoparticles (MAgNPs) covered with single-layer graphene (SLG) are applied as a SERS substrate. The angiotensin converting enzyme 2 (ACE2) is selected as a bio-probes that can specifically bind to the SARS-CoV-2 S protein. The SERS-based biosensor is formed by ACE2 functionalized SLG/MAgNPs and the LODs of detecting SARS-CoV-2 S protein in phosphate-buffered saline (PBS) and in pharyngeal swabs solution (PSS) are 0.1 fg mL and 10 fg mL, respectively. This biosensor provides a way of directly detecting SARS-CoV-2 S protein with high sensitivity and specificity. It illustrates a practical potential in the rapid detection of the SARS-CoV-2 virus.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的全球大流行给经济和人类社会造成了巨大灾难。如今，SARS-CoV-2 正在从人们的记忆中消失，但它仍然存在于我们身边。聚合酶链反应（PCR）在检测 SARS-CoV-2 方面发挥着重要作用，但它需要较长的检测时间、配备实验室和专业操作人员。与聚合酶链反应（PCR）相比，表面增强拉曼散射（SERS）是一种很有前途的 SARS-CoV-2 检测方法，因为它具有快速、易于操作和高灵敏度的特性。在本研究中，单层银纳米颗粒（MAgNPs）覆盖单原子层石墨烯（SLG）被用作 SERS 基底。血管紧张素转化酶 2（ACE2）被选为生物探针，可特异性结合 SARS-CoV-2 S 蛋白。基于 SERS 的生物传感器是由 ACE2 功能化的 SLG/MAgNPs 形成的，在磷酸盐缓冲盐水（PBS）和咽拭子溶液（PSS）中检测 SARS-CoV-2 S 蛋白的 LODs 分别为 0.1 fg mL 和 10 fg mL。该生物传感器提供了一种具有高灵敏度和特异性的直接检测 SARS-CoV-2 S 蛋白的方法。它说明了在 SARS-CoV-2 病毒的快速检测方面具有实际潜力。
