Cooper E H, Turner R, Johns E A, Crockson R A
Biomed Pharmacother. 1985;39(2):78-82.
An evaluation of Superose 6, a new high performance gel filtration medium, has been made for the rapid separation and quantitation of paraprotein polymers. There is a significant correlation between relative serum viscosity and the concentration of polymeric IgA as demonstrated by FPLC using the Superose 6 columns. The retention times of the columns are highly reproducible and allow good resolution of polymers and provide a simple way of separating IgG3 from albumin. Scaling up to the preparative Superose 6B could be achieved with little loss of resolution.
对一种新型高效凝胶过滤介质Superose 6进行了评估，用于快速分离和定量副蛋白聚合物。使用Superose 6柱的快速蛋白质液相色谱（FPLC）显示，相对血清粘度与聚合IgA浓度之间存在显著相关性。这些柱的保留时间具有高度可重复性，能很好地分离聚合物，还提供了一种从白蛋白中分离IgG3的简单方法。放大至制备型Superose 6B时分辨率损失很小。
