Andersson T, Hagel L
Anal Biochem. 1984 Sep;141(2):461-5. doi: 10.1016/0003-2697(84)90071-x.
Applications of a new agarose-based medium for high-performance preparative gel filtration is described. The 30-micron bead size, high pore volume, and simple experimental technique to prepare highly efficient columns make Superose 6B very suitable for preparative purifications of protein mixtures in the molar mass range of 10(3) to 4 X 10(6). Sample load exceeds 100 mg/cm2, which indicates that gram quantities may be purified on K 50/60 columns. The scale-up procedure from analytical to preparative columns is demonstrated by the separation of serum samples and the industrial purification of an enzyme-antibody conjugate.
本文描述了一种基于琼脂糖的新型介质在高效制备性凝胶过滤中的应用。30微米的珠粒尺寸、高孔体积以及制备高效柱的简单实验技术，使得Superose 6B非常适合用于分子量范围在10³至4×10⁶的蛋白质混合物的制备性纯化。样品负载量超过100mg/cm²，这表明在K 50/60柱上可以纯化克级量的样品。通过血清样品的分离以及酶-抗体偶联物的工业纯化，展示了从分析柱到制备柱的放大过程。
