Youngman R J, Wagner G R, Kühne F W, Elstner E F
Z Naturforsch C Biosci. 1985 May-Jun;40(5-6):409-14. doi: 10.1515/znc-1985-5-621.
Oxidation of methionine, 4-(methylthio)-2-oxobutyric acid (KMB), or 1-aminocyclopropane carbonic acid (ACC) are indicator reactions for activated oxygen species such as singlet oxygen (1O2), OH.-radical like oxidants, superoxide anion (O2.-), hydrogen peroxide (H2O2) or activated hemo-iron complexes like peroxidase- or catalase-"compound I". Methionine is oxidized by OH. as well as by 1O2 forming ethylene, but not by tetrachloro-decaoxygen complex (TCDO) in the absence or presence of catalytic hemoproteins such as peroxidase, hemoglobin or myoglobin. Both KMB and ACC are oxidized by TCDO under the catalysis of the above hemo-proteins where neither catalase nor superoxide dismutase are inhibitors. TCDO hemo-protein complex is an oxidant with similar properties as peroxidase-compound I and can clearly be differentiated from O2.-, H2O2, OH. and 1O2.
甲硫氨酸、4-(甲硫基)-2-氧代丁酸（KMB）或1-氨基环丙烷羧酸（ACC）的氧化是活性氧物种的指示反应，这些活性氧物种如单线态氧（¹O₂）、类似羟基自由基的氧化剂、超氧阴离子（O₂⁻）、过氧化氢（H₂O₂）或类似过氧化物酶或过氧化氢酶“化合物I”的活化血红素铁配合物。甲硫氨酸可被羟基自由基以及单线态氧氧化形成乙烯，但在不存在或存在催化血红蛋白质如过氧化物酶、血红蛋白或肌红蛋白的情况下，不会被四氯十氧配合物（TCDO）氧化。在上述血红蛋白质的催化下，KMB和ACC均可被TCDO氧化，而过氧化氢酶和超氧化物歧化酶均不是其抑制剂。TCDO-血红蛋白质配合物是一种具有与过氧化物酶-化合物I相似性质的氧化剂，并且可以与超氧阴离子、过氧化氢、羟基自由基和单线态氧明显区分开来。
