Elstner E F, Schütz W, Vogl G
Institute of Botany and Microbiology, Biochemical Laboratory, Technical University of Munich, Federal Republic of Germany.
Arch Toxicol. 1988;62(6):424-7. doi: 10.1007/BF00288344.
Methylthioketobutyric acid has been used as an indicator for the production of reactive oxygen species during incubation with xanthine oxidase or NADH diaphorase in the presence of an autooxidizable quinone. The production of OH-radical-type oxidants is enhanced in the presence of crocidolite but not by the asbestos types chrysotile or amosite. This activity of crocidolite in the diaphorase system is further stimulated by bisulfite. Crocidolite-dependent ethylene formation from methylthioketo-butyric acid is inhibited by both superoxide dismutase and catalase. In the presence of both crocidolite and bisulfite, however, the inhibition by superoxide dismutase is preserved, but the inhibition by catalase is lost. Since in some respect the NADH-diaphorase quinone system may reflect the situation in the activated macrophage, crocidolite activation may represent a biochemical model system describing potential asbestos toxicity.
在可自动氧化的醌存在的情况下，甲硫基酮丁酸已被用作与黄嘌呤氧化酶或NADH黄递酶孵育期间活性氧生成的指示剂。在青石棉存在下，OH自由基型氧化剂的生成会增强，但温石棉或铁石棉类型的石棉则不会。亚硫酸氢盐会进一步刺激青石棉在黄递酶系统中的这种活性。超氧化物歧化酶和过氧化氢酶均会抑制青石棉依赖的由甲硫基酮丁酸生成乙烯的过程。然而，在同时存在青石棉和亚硫酸氢盐的情况下，超氧化物歧化酶的抑制作用仍然存在，但过氧化氢酶的抑制作用消失。由于在某些方面，NADH黄递酶醌系统可能反映活化巨噬细胞中的情况，因此青石棉活化可能代表一个描述潜在石棉毒性的生化模型系统。
