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振动加速度增强 C2C12 细胞的增殖、迁移和成熟，并促进雄性大鼠肌肉损伤的再生。

Vibration acceleration enhances proliferation, migration, and maturation of C2C12 cells and promotes regeneration of muscle injury in male rats.

机构信息

Department of Sports Medical Science, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

Department of Medicine for Sports and Performing Arts, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

出版信息

Physiol Rep. 2024 Feb;12(4):e15905. doi: 10.14814/phy2.15905.

DOI:10.14814/phy2.15905
PMID:38396237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10890929/
Abstract

Vibration acceleration (VA) using a whole-body vibration device is beneficial for skeletal muscles. However, its effect at the cellular level remains unclear. We aimed to investigate the effects of VA on muscles in vitro and in vivo using the C2C12 mouse myoblast cell line and cardiotoxin-induced injury in male rat soleus muscles. Cell proliferation was evaluated using the WST/CCK-8 assay and proportion of Ki-67 positive cells. Cell migration was assessed using wound-healing assay. Cell differentiation was examined by the maturation index in immunostained cultured myotubes and real-time polymerase chain reaction. Regeneration of soleus muscle in rats was assessed by recruitment of satellite cells, cross-sectional area of regenerated muscle fibers, number of centrally nucleated fibers, and conversion of regenerated muscle from fast- to slow-twitch. VA at 30 Hz with low amplitude for 10 min promoted C2C12 cell proliferation, migration, and myotube maturation, without promoting expression of genes related to differentiation. VA significantly increased Pax7-stained satellite cells and centrally nucleated fibers in injured soleus muscles on Day 7 and promoted conversion of fast- to slow-twitch muscle fibers with an increase in the mean cross-sectional area of regenerated muscle fibers on Day 14. VA enhanced the proliferation, migration, and maturation of C2C12 myoblasts and regeneration of injured rat muscles.

摘要

全身振动设备产生的振动加速度（VA）有益于骨骼肌。然而，其在细胞水平上的作用尚不清楚。我们旨在使用 C2C12 小鼠成肌细胞系和心肌毒素诱导的雄性大鼠比目鱼肌损伤来研究 VA 在体外和体内对肌肉的影响。使用 WST/CCK-8 测定法和 Ki-67 阳性细胞比例评估细胞增殖。通过划痕愈合测定评估细胞迁移。通过培养的肌管免疫染色的成熟指数和实时聚合酶链反应检查细胞分化。通过卫星细胞募集、再生肌纤维的横截面积、中央核纤维的数量以及再生肌肉从快肌向慢肌的转化来评估大鼠比目鱼肌的再生。振幅为 30 Hz、持续时间为 10 分钟的低频 VA 促进了 C2C12 细胞的增殖、迁移和肌管成熟，但不促进与分化相关的基因表达。VA 在第 7 天显著增加了损伤比目鱼肌中 Pax7 染色的卫星细胞和中央核纤维，并在第 14 天促进了快肌向慢肌纤维的转化，同时增加了再生肌纤维的平均横截面积。VA 增强了 C2C12 成肌细胞的增殖、迁移和成熟以及损伤大鼠肌肉的再生。

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