Hund Stella M M, Golde Jonas, Tetschke Florian, Basche Sabine, Meier Melina, Kirsten Lars, Koch Edmund, Hannig Christian, Walther Julia
Department of Medical Physics and Biomedical Engineering, Faculty Carl Gustav Carus of Medicine, TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
Polyclinic of Operative Dentistry, Periodontology and Pediatric Dentistry, Faculty of Medicine Carl Gustav Carus, TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.
Diagnostics (Basel). 2024 Feb 7;14(4):367. doi: 10.3390/diagnostics14040367.
Early caries diagnosis still challenges dentistry. Polarization-sensitive optical coherence tomography (PS-OCT) is promising to detect initial lesions non-invasively in depth-resolved cross-sectional visualization. PS-OCT with determined degree of polarization (DOP) imaging provides an intuitive demineralization contrast. The aim of this study is to evaluate the suitability of DOP-based PS-OCT imaging to monitor controlled de- and remineralization progression for the first time and to introduce it as a valid, non-destructive in vitro detection method. Twelve standardized bovine enamel specimens were divided in different groups and demineralized with hydrochloric acid (HCl) as well as partly remineralized with fluoride over a 14-day pH-cycling experiment. The specimens were stored in artificial saliva and sodium chloride (NaCl), respectively. Progress measurements with PS-OCT were made with polarization-sensitive en faceand B-scan mode for qualitative evaluation. The specimens demineralized in HCl showed the most pronounced surface change (lowest DOP) and the most significant increase in depolarization. Additional fluoride treatment and the storage in artificial saliva resulted in the opposite (highest DOP). Therefore, DOP-based PS-OCT imaging appears to be a valuable technique for visualization and monitoring of enamel demineralization and remineralization processes in vitro. However, these findings need to be confirmed in human teeth ex vivo or in situ.
早期龋齿诊断仍然是牙科领域的一项挑战。偏振敏感光学相干断层扫描（PS-OCT）有望在深度分辨的横截面可视化中无创检测初始病变。具有确定偏振度（DOP）成像的PS-OCT提供了直观的脱矿质对比度。本研究的目的是首次评估基于DOP的PS-OCT成像监测可控脱矿质和再矿化进展的适用性，并将其作为一种有效的非破坏性体外检测方法引入。在为期14天的pH循环实验中，将12个标准化的牛牙釉质标本分成不同组，用盐酸（HCl）进行脱矿质处理，并部分用氟化物进行再矿化处理。标本分别保存在人工唾液和氯化钠（NaCl）中。使用偏振敏感的面内成像和B扫描模式通过PS-OCT进行进展测量，以进行定性评估。在HCl中脱矿质的标本显示出最明显的表面变化（最低的DOP）和去极化的最显著增加。额外的氟化物处理和保存在人工唾液中则产生相反的结果（最高的DOP）。因此，基于DOP的PS-OCT成像似乎是一种用于体外可视化和监测牙釉质脱矿质和再矿化过程的有价值技术。然而，这些发现需要在离体或原位的人牙中得到证实。
