Hurtado-Morillas Clara, Martínez-Rodrigo Abel, Orden José A, de Urbina-Fuentes Laura, Mas Alicia, Domínguez-Bernal Gustavo
INMIVET, Animal Health Department, School of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain.
INMIVET, Animal Science Department, School of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, Spain.
Animals (Basel). 2024 Feb 13;14(4):605. doi: 10.3390/ani14040605.
Canine leishmaniosis (CanL) is a growing health problem for which vaccination is a crucial tool for the control of disease. The successful development of an effective vaccine against this disease relies on eliciting a robust and enduring T-cell immune response involving the activation of CD4 Th1 and CD8 T-cells. This study aimed to evaluate the immunogenicity and prophylactic efficacy of a novel nanovaccine comprising a multi-epitope peptide, known as HisDTC, encapsulated in PLGA nanoparticles against infection in the murine model. The encapsulation strategy was designed to enhance antigen loading and sustain release, ensuring prolonged exposure to the immune system. Our results showed that mice immunized with PLGA-encapsulated HisDTC exhibited a significant reduction in the parasite load in the liver and spleen over both short and long-term duration. This reduction was associated with a cellular immune profile marked by elevated levels of pro-inflammatory cytokines, such as IFN-γ, and the generation of memory T cells. In conclusion, the current study establishes that PLGA-encapsulated HisDTC can promote effective and long-lasting T-cell responses against in the murine model. These findings underscore the potential utility of multi-epitope vaccines, in conjunction with appropriate delivery systems, as an alternative strategy for CanL control.
犬利什曼病（CanL）是一个日益严重的健康问题，疫苗接种是控制该疾病的关键工具。开发一种有效的针对这种疾病的疫苗，成功与否依赖于引发强大且持久的T细胞免疫反应，其中涉及CD4 Th1和CD8 T细胞的激活。本研究旨在评估一种新型纳米疫苗的免疫原性和预防效果，该纳米疫苗由包裹在聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒中的多表位肽HisDTC组成，用于小鼠模型中的感染预防。这种包裹策略旨在提高抗原负载量并实现持续释放，确保免疫系统长时间接触抗原。我们的结果表明，用PLGA包裹的HisDTC免疫的小鼠在短期和长期内，肝脏和脾脏中的寄生虫载量均显著降低。这种降低与以促炎细胞因子如干扰素-γ水平升高以及记忆T细胞产生为特征的细胞免疫谱相关。总之，当前研究证实，PLGA包裹的HisDTC可在小鼠模型中促进针对感染的有效且持久的T细胞反应。这些发现强调了多表位疫苗结合适当的递送系统作为CanL控制替代策略的潜在效用。
