Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
Int J Mol Sci. 2024 Feb 7;25(4):2045. doi: 10.3390/ijms25042045.
The water and ethanol extracts of huangqin, the roots of Georgi. with potential antiviral properties and antioxidant activities, were investigated for their chemical profiles and their abilities to interfere with the interaction between SARS-CoV-2 spike protein and ACE2, inhibiting ACE2 activity and scavenging free radicals. A total of 76 compounds were tentatively identified from the extracts. The water extract showed a greater inhibition on the interaction between SARS-CoV-2 spike protein and ACE2, but less inhibition on ACE2 activity than that of the ethanol extract on a per botanical weight concentration basis. The total phenolic content was 65.27 mg gallic acid equivalent (GAE)/g dry botanical and the scavenging capacities against HO, DPPH, and ABTS were 1369.39, 334.37, and 533.66 µmol trolox equivalent (TE)/g dry botanical for the water extract, respectively. These values were greater than those of the ethanol extract, with a TPC of 20.34 mg GAE/g, and 217.17, 10.93, and 50.21 µmol TE/g against HO, DPPH, and ABTS, respectively. The results suggested the potential use of huangqin as a functional food ingredient in preventing COVID-19.
黄芩的水提物和乙醇提取物具有潜在的抗病毒特性和抗氧化活性，研究了它们的化学成分以及它们干扰 SARS-CoV-2 刺突蛋白与 ACE2 相互作用、抑制 ACE2 活性和清除自由基的能力。从提取物中初步鉴定出 76 种化合物。水提取物在抑制 SARS-CoV-2 刺突蛋白与 ACE2 相互作用方面的效果优于乙醇提取物，但在抑制 ACE2 活性方面的效果不如乙醇提取物。水提取物的总酚含量为 65.27mg 没食子酸当量（GAE）/g 干植物，对 HO、DPPH 和 ABTS 的清除能力分别为 1369.39、334.37 和 533.66µmol trolox 当量（TE）/g 干植物。这些值大于乙醇提取物的值，其 TPC 为 20.34mg GAE/g，对 HO、DPPH 和 ABTS 的清除能力分别为 217.17、10.93 和 50.21µmol TE/g。结果表明，黄芩有作为预防 COVID-19 的功能性食品成分的潜力。
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