培养中的恶性疟原虫：改良的连续流动法

Plasmodium falciparum in culture: improved continuous flow method.

作者信息

Trager W

出版信息

J Protozool. 1979 Feb;26(1):125-9. doi: 10.1111/j.1550-7408.1979.tb02747.x.

DOI:10.1111/j.1550-7408.1979.tb02747.x

Abstract

A new design of flow vessel provides a method for continuous culture of P. falciparum in a settled layer of human erythrocytes with a slow flow of culture medium over them. The parasitemia is kept fluctuating from approximately 1%, just after addition of fresh erythrocytes, to approximately 10%, 2 or 3 days later. Each vessel provides each week 3 harvests, each containing approximately 0.6--1 X 10(9) parasites.

摘要

一种新型流动培养容器提供了一种在人红细胞沉降层中连续培养恶性疟原虫的方法，培养基在其上缓慢流动。疟原虫血症在刚添加新鲜红细胞后约为1%，2或3天后波动至约10%。每个容器每周可收获3次，每次收获约含0.6 - 1×10⁹个疟原虫。