Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow, Gronostajowa 9, 30-387 Krakow, Poland.
Int J Mol Sci. 2024 Feb 16;25(4):2339. doi: 10.3390/ijms25042339.
Visfatin/NAMPT (VIS), the hormone exerting a pleiotropic effect, is also perceived as an important factor in the regulation of reproductive processes and pregnancy maintenance. Previous studies confirmed its involvement in the control of porcine pituitary and ovary function. In this study, we hypothesized that VIS may affect the global transcriptome of luteal cells and thus regulate the functioning of the ovaries. Illumina's NovaSeq 6000 RNA sequencing was performed to investigate the differentially expressed genes (DEGs) and long non-coding RNAs (DELs) as well as the occurrence of differential alternative splicing events (DASs) in the porcine luteal cells exposed to VIS (100 ng/mL) during the implantation period. The obtained results revealed 170 DEGs (99 up- and 71 downregulated) assigned to 45 functional annotations. Moreover, we revealed 40 DELs, of which 3 were known and 37 were described for the first time. We identified 169 DASs events. The obtained results confirmed a significant effect of VIS on the transcriptome and spliceosome of luteal cells, including the genes involved in the processes crucial for successful implantation and pregnancy maintenance as angiogenesis, steroidogenesis, inflammation, cell development, migration, and proliferation.
内脂素/NAMPT（VIS）是一种具有多种生物学效应的激素，也被认为是调节生殖过程和维持妊娠的重要因素。先前的研究证实了它参与了猪垂体和卵巢功能的控制。在这项研究中，我们假设 VIS 可能影响黄体细胞的全转录组，从而调节卵巢的功能。使用 Illumina 的 NovaSeq 6000 RNA 测序技术，研究了在植入期暴露于 VIS（100ng/ml）的猪黄体细胞中差异表达的基因（DEGs）和长非编码 RNA（DELs）以及差异剪接事件（DASs）的发生情况。结果显示，有 170 个 DEGs（99 个上调和 71 个下调）被分配到 45 个功能注释中。此外，我们还发现了 40 个 DEL，其中 3 个是已知的，37 个是首次描述的。我们鉴定了 169 个 DASs 事件。研究结果证实了 VIS 对黄体细胞转录组和剪接体的显著影响，包括参与成功植入和妊娠维持的关键过程的基因，如血管生成、类固醇生成、炎症、细胞发育、迁移和增殖。
