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内脂素（烟酰胺磷酸核糖转移酶）在发情周期的黄体中期对猪垂体前叶细胞的整体基因表达产生影响。

Visfatin (NAMPT) affects global gene expression in porcine anterior pituitary cells during the mid-luteal phase of the oestrous cycle.

作者信息

Dobrzyn Kamil, Kopij Grzegorz, Kiezun Marta, Zaobidna Ewa, Gudelska Marlena, Zarzecka Barbara, Paukszto Lukasz, Rak Agnieszka, Smolinska Nina, Kaminski Tadeusz

机构信息

Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.

出版信息

J Anim Sci Biotechnol. 2024 Jul 9;15(1):96. doi: 10.1186/s40104-024-01054-z.

DOI:10.1186/s40104-024-01054-z
PMID:38978053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11232246/
Abstract

BACKGROUND

The pituitary belongs to the most important endocrine glands involved in regulating reproductive functions. The proper functioning of this gland ensures the undisturbed course of the oestrous cycle and affects the female's reproductive potential. It is believed that visfatin, a hormone belonging to the adipokine family, may regulate reproductive functions in response to the female's metabolic state. Herein we verified the hypothesis that suggests a modulatory effect of visfatin on the anterior pituitary transcriptome during the mid-luteal phase of the oestrous cycle.

RESULTS

RNA-seq analysis of the porcine anterior pituitary cells revealed changes in the expression of 202 genes (95 up-regulated and 107 down-regulated in the presence of visfatin, when compared to the non-treated controls), assigned to 318 gene ontology terms. We revealed changes in the frequency of alternative splicing events (235 cases), as well as long noncoding RNA expression (79 cases) in the presence of the adipokine. The identified genes were associated, among others, with reproductive system development, epithelial cell proliferation, positive regulation of cell development, gland morphogenesis and cell chemotaxis.

CONCLUSIONS

The obtained results indicate a modulatory influence of visfatin on the regulation of the porcine transcriptome and, in consequence, pituitary physiology during the mid-luteal phase of the oestrous cycle.

摘要

背景

垂体是参与调节生殖功能的最重要内分泌腺之一。该腺体的正常功能确保发情周期的顺利进行，并影响雌性的生殖潜力。据信，内脂素是一种属于脂肪因子家族的激素，可能根据雌性的代谢状态调节生殖功能。在此，我们验证了一个假说，即内脂素在发情周期的黄体中期对垂体前叶转录组具有调节作用。

结果

对猪垂体前叶细胞的RNA测序分析显示，有202个基因的表达发生了变化（与未处理的对照组相比，在内脂素存在的情况下，95个基因上调，107个基因下调），这些基因被归入318个基因本体术语。我们发现，在脂肪因子存在的情况下，可变剪接事件的频率（235例）以及长链非编码RNA的表达（79例）都发生了变化。所鉴定的基因与生殖系统发育、上皮细胞增殖、细胞发育的正向调节、腺体形态发生和细胞趋化性等有关。

结论

获得的结果表明，内脂素在发情周期的黄体中期对猪转录组的调节以及垂体生理学具有调节作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/7c4d2e1dbe0c/40104_2024_1054_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/582520763426/40104_2024_1054_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/77dbcaf0be4a/40104_2024_1054_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/ae38ffd39d40/40104_2024_1054_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/5f7a1aca6b1d/40104_2024_1054_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/0e5d2ef67027/40104_2024_1054_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/813a87c49b70/40104_2024_1054_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/ad9db4c58137/40104_2024_1054_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/1c086dc98e4c/40104_2024_1054_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/7c4d2e1dbe0c/40104_2024_1054_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/582520763426/40104_2024_1054_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/77dbcaf0be4a/40104_2024_1054_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/ae38ffd39d40/40104_2024_1054_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/5f7a1aca6b1d/40104_2024_1054_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/0e5d2ef67027/40104_2024_1054_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/813a87c49b70/40104_2024_1054_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/ad9db4c58137/40104_2024_1054_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/1c086dc98e4c/40104_2024_1054_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3592/11232246/7c4d2e1dbe0c/40104_2024_1054_Fig9_HTML.jpg

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Cells. 2023 Dec 14;12(24):2835. doi: 10.3390/cells12242835.
2
Visfatin in the porcine pituitary gland: expression and regulation of secretion during the oestrous cycle and early pregnancy.猪脑垂体中的内脂素：发情周期和早孕期间分泌的表达和调节。
Sci Rep. 2023 Oct 25;13(1):18253. doi: 10.1038/s41598-023-45255-4.
3
Therapeutic gene silencing of leads to lethality in genetically unstable cancer cells.
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Sci Adv. 2023 Apr 5;9(14):eade4800. doi: 10.1126/sciadv.ade4800.
4
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Cancer Res. 2022 Oct 17;82(20):3734-3750. doi: 10.1158/0008-5472.CAN-22-1695.
5
Chemerin effect on transcriptome of the porcine endometrium during implantation determined by RNA-sequencing†.通过 RNA 测序研究 chemerin 对猪植入期子宫内膜转录组的影响†。
Biol Reprod. 2022 Aug 9;107(2):557-573. doi: 10.1093/biolre/ioac063.
6
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Cell Rep. 2021 Oct 12;37(2):109815. doi: 10.1016/j.celrep.2021.109815.
7
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