College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2024 Jan 30;15(2):185. doi: 10.3390/genes15020185.
is a frequently cultivated ornamental plant containing several secondary metabolites, including alkaloids, flavonoids, and volatile organic compounds (VOCs). However, extensive research has been conducted only on non-VOCs found in the plant, whereas the production of VOCs and the molecular mechanisms underlying the biosynthesis of terpenes remain poorly understood. In this study, 17 volatile compounds were identified from flowers using gas chromatography-mass spectrometry (GC-MS), with 16 of them being terpenoids. Transcriptome sequencing resulted in the identification of 17 terpene synthase () genes; two genes, ZcTPS01 and ZcTPS02, had high expression levels. Biochemical characterization of two enzymes encoded by both genes revealed that can catalyze geranyl diphosphate (GPP) into diverse products, among which is -ocimene, which is the second most abundant compound found in flowers. These results suggest that plays a vital role in -ocimene biosynthesis, providing valuable insights into terpene biosynthesis pathways in . Furthermore, the expression of ZcTPS02 was upregulated after 2 h of methyl jasmonate (MeJA) treatment and downregulated after 4 h of the same treatment.
是一种经常被栽培的观赏植物，含有几种次生代谢产物，包括生物碱、类黄酮和挥发性有机化合物（VOCs）。然而，人们仅对该植物中发现的非-VOCs 进行了广泛的研究，而 VOCs 的产生以及萜类化合物生物合成的分子机制仍知之甚少。在这项研究中，使用气相色谱-质谱联用（GC-MS）从花朵中鉴定出 17 种挥发性化合物，其中 16 种为萜类化合物。转录组测序鉴定出了 17 个萜烯合酶（）基因；两个基因，ZcTPS01 和 ZcTPS02，表达水平较高。对这两个基因编码的两种酶进行的生化特性分析表明，能够催化香叶基二磷酸（GPP）生成多种产物，其中包括-罗勒烯，这是在 花朵中发现的第二丰富的化合物。这些结果表明，在 -罗勒烯生物合成中发挥着重要作用，为 中的萜类化合物生物合成途径提供了有价值的见解。此外，ZcTPS02 的表达在茉莉酸甲酯（MeJA）处理 2 小时后上调，而在相同处理 4 小时后下调。
