Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450003, China.
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed by Henan Province & Education Ministry of China, Zhengzhou 450046, China.
Molecules. 2024 Feb 7;29(4):770. doi: 10.3390/molecules29040770.
The objective of this study was to identify and evaluate the pharmacodynamic constituents of Ardisiae Japonicae Herba (AJH) for the treatment of acute lung injury (ALI). To fully analyze the chemical contents of various extraction solvents (petroleum ether site (PE), ethyl acetate site (EA), -butanol site (NB), and water site (WS)) of AJH, the UPLC-Orbitrap Fusion-MS technique was employed. Subsequently, the anti-inflammatory properties of the four extracted components of AJH were assessed using the lipopolysaccharide (LPS)-induced MH-S cellular inflammation model. The parts that exhibited anti-inflammatory activity were identified. Additionally, a technique was developed to measure the levels of specific chemical constituents in the anti-inflammatory components of AJH. The correlation between the "anti-inflammatory activity" and the constituents was analyzed, enabling the identification of a group of pharmacodynamic components with anti-inflammatory properties. ALI model rats were created using the tracheal drip LPS technique. The pharmacodynamic indices were evaluated for the anti-inflammatory active portions of AJH. The research revealed that the PE, EA, NB, and WS extracts of AJH included 215, 289, 128, and 69 unique chemical components, respectively. Additionally, 528 chemical components were discovered after removing duplicate values from the data. The EA exhibited significant anti-inflammatory activity in the cellular assay. A further analysis was conducted to determine the correlation between anti-inflammatory activity and components. Seventeen components, such as caryophyllene oxide, bergenin, and gallic acid, were identified as potential pharmacodynamic components with anti-inflammatory activity. The pharmacodynamic findings demonstrated that the intermediate and high doses of the EA extract from AJH exhibited a more pronounced effect in enhancing lung function, blood counts, and lung histology in a way that depended on the dosage. To summarize, when considering the findings from the previous study on the chemical properties of AJH, it was determined that the EA contained a group of 13 constituents that primarily contributed to its pharmacodynamic effects against ALI. The constituents include bergenin, quercetin, epigallocatechingallate, and others.
本研究旨在鉴定和评价紫金牛属草药（AJH）治疗急性肺损伤（ALI）的药效成分。为了全面分析 AJH 不同提取溶剂（石油醚部位（PE）、乙酸乙酯部位（EA）、正丁醇部位（NB）和水部位（WS））的化学成分含量，采用 UPLC-Orbitrap Fusion-MS 技术。随后，采用脂多糖（LPS）诱导的 MH-S 细胞炎症模型评估 AJH 四种提取物的抗炎特性。鉴定出具有抗炎活性的部分。此外，还开发了一种测量 AJH 抗炎成分中特定化学成分含量的技术。分析了“抗炎活性”与成分之间的相关性，确定了一组具有抗炎作用的药效成分。采用气管滴注 LPS 技术建立 ALI 模型大鼠，评价 AJH 抗炎活性部位的药效学指标。研究发现，AJH 的 PE、EA、NB 和 WS 提取物分别包含 215、289、128 和 69 种独特的化学成分，此外，数据去除重复值后发现了 528 种化学成分。细胞实验表明 EA 具有显著的抗炎活性。进一步分析抗炎活性与成分之间的相关性。鉴定出 17 种潜在的具有抗炎活性的药效成分，如石竹烯氧化物、 Bergenin 和没食子酸等。药效学研究结果表明，AJH 的 EA 提取物中剂量和高剂量在增强肺功能、血液计数和肺组织学方面的作用更为明显，这取决于剂量。总之，考虑到之前 AJH 化学性质研究的结果，确定 EA 含有一组 13 种主要发挥药效作用的成分，对抗 ALI。这些成分包括 Bergenin、槲皮素、表没食子儿茶素没食子酸酯等。
