新型野生型菌株作为管理肥胖相关胰岛素抵抗的益生菌候选菌株。

Novel Wild-Type and Strains as Probiotic Candidates to Manage Obesity-Associated Insulin Resistance.

作者信息

Somalou Paraskevi, Ieronymaki Eleftheria, Feidaki Kyriaki, Prapa Ioanna, Stylianopoulou Electra, Spyridopoulou Katerina, Skavdis George, Grigoriou Maria E, Panas Panayiotis, Argiriou Anagnostis, Tsatsanis Christos, Kourkoutas Yiannis

机构信息

Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.

Laboratory of Clinical Chemistry, Department of Laboratory Medicine, Medical School, University of Crete, 71003 Crete, Greece.

出版信息

Microorganisms. 2024 Jan 23;12(2):231. doi: 10.3390/microorganisms12020231.

DOI:10.3390/microorganisms12020231

PMID:38399636

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10891751/

Abstract

As the food and pharmaceutical industry is continuously seeking new probiotic strains with unique health properties, the aim of the present study was to determine the impact of short-term dietary intervention with novel wild-type strains, isolated from various sources, on high-fat diet (HFD)-induced insulin resistance. Initially, the strains were evaluated in vitro for their ability to survive in simulated gastrointestinal (GI) conditions, for adhesion to Caco-2 cells, for bile salt hydrolase secretion, for cholesterol-lowering and cellular cholesterol-binding ability, and for growth inhibition of food-borne pathogens. In addition, safety criteria were assessed, including hemolytic activity and susceptibility to antibiotics. The in vivo test on insulin resistance showed that mice receiving the HFD supplemented with SK (isolated from human feces) or OLS3-1 strain (isolated from olive fruit) exhibited significantly improved insulin resistance compared to HFD-fed mice or to the normal diet (ND)-fed group.

摘要

由于食品和制药行业不断寻求具有独特健康特性的新型益生菌菌株，本研究的目的是确定用从各种来源分离出的新型野生型菌株进行短期饮食干预对高脂饮食（HFD）诱导的胰岛素抵抗的影响。最初，对这些菌株进行了体外评估，包括它们在模拟胃肠道（GI）条件下的存活能力、对Caco-2细胞的粘附能力、胆盐水解酶分泌能力、降胆固醇和细胞胆固醇结合能力，以及对食源性病原体的生长抑制能力。此外，还评估了安全性标准，包括溶血活性和对抗生素的敏感性。关于胰岛素抵抗的体内试验表明，与高脂饮食喂养的小鼠或正常饮食（ND）喂养组相比，接受补充了SK（从人类粪便中分离）或OLS3-1菌株（从橄榄果实中分离）的高脂饮食的小鼠的胰岛素抵抗有显著改善。