在开心果上用游离或固定化SK细胞进行饮食干预后，糖尿病大鼠肠道微生物群和血液参数的功能调节

Functional Modulation of Gut Microbiota and Blood Parameters in Diabetic Rats Following Dietary Intervention with Free or Immobilized SK Cells on Pistachio Nuts.

作者信息

Prapa Ioanna, Yanni Amalia E, Kompoura Vasiliki, Mitropoulou Gregoria, Panas Panayiotis, Kostomitsopoulos Nikolaos, Kourkoutas Yiannis

机构信息

Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece.

Laboratory of Chemistry, Biochemistry, Physical Chemistry of Foods, Department of Nutrition and Dietetics, Harokopio University of Athens, 17671 Athens, Greece.

出版信息

Nutrients. 2024 Dec 6;16(23):4221. doi: 10.3390/nu16234221.

DOI:10.3390/nu16234221

PMID:39683613

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11644729/

Abstract

BACKGROUND/OBJECTIVES: The gut microbiota is linked to the pathogenesis of type 1 diabetes mellitus (T1DM), while supplementation with probiotics may result in positive alterations in the composition of the gut microbiome. This research aimed to map the changes in the gut microbiome and blood markers of streptozotocin-induced diabetic rats after a dietary intervention with free or immobilized cells of the presumptive probiotic SK on pistachio nuts.

METHODS

Twenty-four male Wistar rats were studied and divided into four groups (healthy or diabetic) which received the free or the immobilized . SK cells on pistachio nuts for 4 weeks. Blood, fecal, and intestinal tissue samples were examined.

RESULTS

The diabetic rats exhibited an elevated concentration of HDL-c, while the inflammatory IL-1β levels were significantly lower in the diabetic animals that received the immobilized cells compared to the group that received the free cells. The dietary intervention with immobilized cells led to decreased counts of fecal staphylococci and enterococci in the diabetic animals, while the diet with both free and immobilized . SK cells rendered levels of these populations in normal values in the feces and intestinal tissue of the diabetic animals. Noticeably, the and genera were elevated after the supplementation with immobilized SK cells on pistachio nuts.

CONCLUSIONS

Dietary supplementation with SK cells (in free or in immobilized form) beneficially affected the gut microbiota/microbiome of streptozotocin-induced diabetic rats, leading to the alleviation of dysbiosis and inflammation and control over their lipid levels.

摘要

背景/目的：肠道微生物群与1型糖尿病（T1DM）的发病机制有关，而补充益生菌可能会使肠道微生物组的组成发生积极变化。本研究旨在描绘用开心果上自由或固定化的假定益生菌SK细胞进行饮食干预后，链脲佐菌素诱导的糖尿病大鼠肠道微生物组和血液标志物的变化。

方法

对24只雄性Wistar大鼠进行研究，分为四组（健康或糖尿病组），分别接受开心果上自由或固定化的SK细胞，为期4周。对血液、粪便和肠道组织样本进行检测。

结果

糖尿病大鼠的高密度脂蛋白胆固醇（HDL-c）浓度升高，而与接受自由细胞的组相比，接受固定化细胞的糖尿病动物体内炎症性白细胞介素-1β水平显著降低。用固定化细胞进行饮食干预导致糖尿病动物粪便中葡萄球菌和肠球菌数量减少，而自由和固定化SK细胞的饮食使糖尿病动物粪便和肠道组织中这些菌群的水平恢复正常。值得注意的是，在开心果上补充固定化SK细胞后，[具体菌属名称未给出]菌属增加。