Yamamoto Y, Niki E, Eguchi J, Kamiya Y, Shimasaki H
Biochim Biophys Acta. 1985 Sep 25;819(1):29-36. doi: 10.1016/0005-2736(85)90192-0.
The oxidation of human and rat erythrocyte ghost membranes by molecular oxygen has been performed in an aqueous suspension at 37 degrees C. A constant rate of oxygen uptake was observed in the presence of radical initiator. alpha-Tocopherol in the membrane suppressed the oxidation and the induction period was clearly observed. alpha-Tocopherol decreased linearly during the induction period and when it was depleted the induction period was over and a rapid oxidation started. The rate of oxidation was proportional to the square root of the rate of initial radical generation. The kinetic chain length, the ratio of the rate of propagation to that of initiation, was long, ranging from 7 to 100. These results indicate that the erythrocyte ghost membranes are oxidized by a free radical chain mechanism by molecular oxygen. Among the fatty acids of membrane lipids, polyunsaturated fatty acids were oxidized exclusively. Proteins as well as polyunsaturated fatty acids were oxidized and the formation of the high- and low-molecular-weight proteins and the decrease of protein bands were observed on gel electrophoresis.
在37℃的水悬浮液中，用分子氧对人和大鼠红细胞血影膜进行了氧化。在自由基引发剂存在的情况下，观察到了恒定的吸氧速率。膜中的α-生育酚抑制了氧化作用，并且清楚地观察到了诱导期。在诱导期，α-生育酚呈线性下降，当它耗尽时，诱导期结束，快速氧化开始。氧化速率与初始自由基产生速率的平方根成正比。动力学链长，即传播速率与引发速率之比，很长，范围从7到100。这些结果表明，红细胞血影膜是通过分子氧的自由基链机制被氧化的。在膜脂的脂肪酸中，多不饱和脂肪酸被优先氧化。蛋白质以及多不饱和脂肪酸被氧化，并且在凝胶电泳上观察到了高分子量和低分子量蛋白质的形成以及蛋白条带的减少。
