State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China.
Biosens Bioelectron. 2024 May 15;252:116137. doi: 10.1016/j.bios.2024.116137. Epub 2024 Feb 17.
Inspired by the programmability and modifiability of nucleic acids, point-of-care (POC) diagnostics for nucleic acid target detection is evolving to become more diversified and intelligent. In this study, we introduce a fluorescent and photothermal dual-mode logic biosensing platform that integrates catalytic hairpin assembly (CHA), toehold-mediated stand displacement reaction (SDR) and a DNA walking machine. Dual identification and signal reporting modules are incorporated into DNA circuits, orchestrated by an AND Boolean logic gate operator and magnetic beads (MBs). In the presence of bispecific microRNAs (miRNAs), the AND logic gate activates, driving the DNA walking machine, and facilitating the collection of hairpin DNA stands modified with FAM fluorescent group and CeO@Au nanoparticles. The CeO@Au nanoparticles, served as a nanozyme, can oxidize TMB into oxidation TMB (TMBox), enabling a near-infrared (NIR) laser-driven photothermal effect following the magnetic separation of MBs. This versatile platform was employed to differentiate between plasma samples from breast cancer patients, lung cancer patients, and healthy donors. The thermometer-readout transducers, derived from the CeO@Au@DNA complexes, provided reliable results, further corroborated by fluorescence assays, enhancing the confidence in the diagnostics compared to singular detection method. The dual-mode logic biosensor can be easily customized to various nucleic acid biomarkers and other POC signal readout modalities by adjusting recognition sequences and modification strategies, heralding a promising future in the development of intelligent, flexible diagnostics for POC testing.
受核酸可编程性和可修饰性的启发，用于核酸靶标检测的即时诊断（POC）正在朝着更加多样化和智能化的方向发展。在本研究中，我们引入了一种荧光和光热双模逻辑生物传感平台，该平台集成了催化发夹组装（CHA）、适体引发的链位移反应（SDR）和 DNA 行走机器。双识别和信号报告模块被整合到 DNA 电路中，由 AND 布尔逻辑门操作器和磁珠（MBs）协调。在双特异性 microRNAs（miRNAs）存在的情况下，AND 逻辑门激活，驱动 DNA 行走机器，促进带有 FAM 荧光基团和 CeO@Au 纳米颗粒修饰的发夹 DNA 支架的收集。CeO@Au 纳米颗粒作为纳米酶，可以将 TMB 氧化成氧化 TMB（TMBox），在磁分离 MBs 后，通过近红外（NIR）激光驱动光热效应。该多功能平台用于区分乳腺癌患者、肺癌患者和健康供体的血浆样本。温度计读出传感器，源自 CeO@Au@DNA 复合物，提供了可靠的结果，通过荧光分析进一步证实，与单一检测方法相比，提高了诊断的可信度。通过调整识别序列和修饰策略，双模逻辑生物传感器可以轻松定制用于各种核酸生物标志物和其他 POC 信号读出模式，为 POC 测试的智能、灵活诊断的发展带来了广阔的前景。
