Jain S K, Barrett K J, Boyd D, Favreau M F, Crampton J, Drysdale J W
J Biol Chem. 1985 Sep 25;260(21):11762-8.
This paper explores the complexity of human ferritin H and L genes. We show that essentially full-length cDNA clones for human ferritin H and L chains do not cross-hybridize under moderate stringency conditions and present the first comparisons of H and L mRNAs and genes from the same species. Northern blot analyses indicate that the H and L mRNAs each contain about 1100 nucleotides. Subprobe analyses of Southern blots show that both H and L genes exist as multiple gene families. Both the 5' and 3' sequences of the H genes are heterogeneous, whereas the 3' end of the L gene is relatively conserved.
本文探讨了人类铁蛋白H和L基因的复杂性。我们发现，在中等严格条件下，人类铁蛋白H链和L链的基本全长cDNA克隆不会交叉杂交，并首次对来自同一物种的H和L mRNA及基因进行了比较。Northern印迹分析表明，H和L mRNA各自包含约1100个核苷酸。Southern印迹的亚探针分析表明，H和L基因均以多个基因家族的形式存在。H基因的5'和3'序列都是异质的，而L基因的3'端相对保守。
