人类铁蛋白重链基因的克隆、特性分析、表达及染色体定位

Hentze M W, Keim S, Papadopoulos P, O'Brien S, Modi W, Drysdale J, Leonard W J, Harford J B, Klausner R D

Proc Natl Acad Sci U S A. 1986 Oct;83(19):7226-30. doi: 10.1073/pnas.83.19.7226.

A genomic phage clone containing a full-length copy of a functional human gene for ferritin heavy chain has been isolated. The gene consists of four exons spanning approximately 3 kilobases and has been localized to chromosome 11. The functionality of the gene was demonstrated by the fact that both transient transfectants and stable transformants of murine fibroblasts actively transcribe human ferritin heavy-chain mRNA.

一个包含功能性人铁蛋白重链基因全长拷贝的基因组噬菌体克隆已被分离出来。该基因由四个外显子组成，跨度约为3千碱基，定位于11号染色体。该基因的功能性通过以下事实得以证明：小鼠成纤维细胞的瞬时转染子和稳定转化子都能积极转录人铁蛋白重链mRNA。