Pavlok A, Fléchon J E
J Reprod Fertil. 1985 Jul;74(2):597-604. doi: 10.1530/jrf.0.0740597.
Variability in the interaction of ram spermatozoa with zona-free hamster eggs was recorded not only amongst individual males but also between the first and second ejaculates of the same male collected 30 min apart. Fusion ability also differed according to the conditions of gamete mixing. This ability decreased after in-vitro storage of undiluted ejaculates at room temperature but lasted for 48-192 h. The kinetics of sperm-egg fusion during the time of gamete incubation varied not only with the time of sperm storage in vitro but also with the ejaculate. When the semen was frozen, the ability of the spermatozoa to fuse was markedly reduced.
研究记录到，不仅不同公羊的精子与去透明带仓鼠卵的相互作用存在差异，而且同一公羊间隔30分钟采集的第一次和第二次射精的精子之间也存在差异。融合能力也因配子混合条件而异。未稀释的射精精液在室温下体外储存后，这种能力会下降，但可持续48 - 192小时。配子孵育期间精卵融合的动力学不仅随精子体外储存时间而变化，也随射精情况而变化。精液冷冻后，精子的融合能力明显降低。
