Serafini P C, Hauser D, Moyer D, Marrs R P
Fertil Steril. 1986 Oct;46(4):691-5. doi: 10.1016/s0015-0282(16)49650-0.
Freezing and storage of human male gametes is associated with a reduction in the overall semen quality and establishment of pregnancy. This study was done to evaluate the integrity of sperm head ultrastructure (SHU) with computerized and vapor freezing. Comparisons were made between the effect of cryopreservatives glycerol (G) and dimethylsulfoxide (DMSO) on SHU. Twelve ejaculates from five proven fertile donors were studied with the use of routine semen analysis, zona-free hamster ova, and SHU. Both cooling processes, regardless of the preservative used, significantly reduced sperm function and the number of SH with intact plasma membranes. The staged cooling technique was substantially superior to vapor freezing in all parameters analyzed (P less than 0.01). G was less detrimental to the postthaw SHU than 1 M DMSO. A significant positive correlation (r = 0.98; P less than 0.01) was noted between the total number of intact SH and motile sperm. Computerized freezing in a G-diluted semen rendered a sperm environment that allowed the highest number of forms with intact SH membranes and with the best chances to penetrate zona-free hamster ova.
人类男性配子的冷冻和储存与精液整体质量下降及受孕成功率降低有关。本研究旨在评估计算机控制冷冻和气相冷冻对精子头部超微结构（SHU）完整性的影响。比较了冷冻保护剂甘油（G）和二甲基亚砜（DMSO）对SHU的影响。对来自5名经证实具有生育能力的供体的12份精液样本进行了常规精液分析、去透明带仓鼠卵穿透试验及SHU研究。无论使用哪种冷冻保护剂，两种冷冻过程均显著降低了精子功能及具有完整质膜的SH数量。在所有分析参数中，分步冷却技术均显著优于气相冷冻（P<0.01）。相较于1M DMSO，G对解冻后SHU的损害更小。完整SH总数与活动精子数之间存在显著正相关（r = 0.98；P<0.01）。在G稀释的精液中进行计算机控制冷冻可营造一种精子环境，使具有完整SH膜且穿透去透明带仓鼠卵几率最高的精子形态数量最多。
