Department of Neurosurgery, Huang Shi Central Hospital, HuangShi, China.
Department of Geriatrics, Huang Shi Central Hospital, HuangShi, China.
Neurol Res. 2024 Apr;46(4):346-355. doi: 10.1080/01616412.2024.2321014. Epub 2024 Feb 25.
Neuroinflammation and oxidative stress are critical players in intracerebral hemorrhage (ICH). Geniposide is an active component of Gardenia that has anti-inflammatory effects. This study focused on the roles and mechanisms of geniposide in ICH.
ICH was established by injecting collagenase IV into C57BL/6 mice. To determine the functions of geniposide and NF-κB inhibition in ICH model mice, geniposide (1, 25, or 50 mg/kg) or PDTC (a NF-κB inhibitor) was administered. Neurological functions were assessed with the modified neurological severity score (mNSS) test. Hematoxylin and eosin staining were performed to identify pathological changes. IL-1β and TNF-α levels were estimated with ELISA kits. NF-κB p65 localization was determined by immunofluorescence staining. Oxidative stress was analyzed by measuring ROS levels.
Geniposide alleviated cerebral edema and neurological deficits. Geniposide inhibited neuroinflammation and oxidative stress after ICH, and the inhibitory effects were enhanced by NF-κB inhibition. Additionally, geniposide inhibited NF-κB signaling.
Geniposide alleviates brain injury by suppressing inflammation and oxidative stress damage in experimental ICH models by inhibiting NF-κB signaling.
神经炎症和氧化应激是脑出血（ICH）的关键因素。栀子苷是栀子的一种活性成分，具有抗炎作用。本研究聚焦于栀子苷在 ICH 中的作用和机制。
通过向 C57BL/6 小鼠脑内注射胶原酶 IV 建立 ICH 模型。为了确定栀子苷和 NF-κB 抑制剂在 ICH 模型小鼠中的作用，给予栀子苷（1、25 或 50mg/kg）或 PDTC（NF-κB 抑制剂）。采用改良神经功能缺损评分（mNSS）试验评估神经功能。通过苏木精-伊红染色观察病理变化。采用 ELISA 试剂盒测定 IL-1β 和 TNF-α 水平。通过免疫荧光染色检测 NF-κB p65 定位。通过检测 ROS 水平分析氧化应激。
栀子苷减轻脑水肿和神经功能缺损。栀子苷抑制 ICH 后神经炎症和氧化应激，NF-κB 抑制增强其抑制作用。此外，栀子苷抑制 NF-κB 信号通路。
栀子苷通过抑制 NF-κB 信号通路抑制炎症和氧化应激损伤，从而减轻实验性 ICH 模型中的脑损伤。
