Bigner S H, Mark J, Schold S C, Eng L F, Bigner D D
Cancer Genet Cytogenet. 1985 Oct;18(2):141-53. doi: 10.1016/0165-4608(85)90064-0.
We have karyotyped a human giant cell glioblastoma removed from an 11-year-old girl and have established from it a subcutaneously transplantable line in athymic nude mice. The original tumor contained near-haploid cells with 25 or 26 chromosomes, including two copies of #1, (7 or 7p+) and #18. There were also hyperdiploid (49-52) cells that were tetraploid for these same three chromosome types; doubled versions of the hyperdiploid population were also seen. The stemline of the mouse-grown tumor was 26,X, +1, +7p+, +18 in the first passage and has remained consistently near-haploid through ten serial in vivo passages. Growth stabilization has occurred with an average latency of less than 3 months. This transplantable line is available for evaluating chemotherapeutic responsiveness of human giant cell glioblastoma and for studying near-haploidy in solid human tumors.
我们对一名11岁女孩身上切除的人类巨细胞胶质母细胞瘤进行了核型分析，并从中建立了一个可在无胸腺裸鼠皮下移植的细胞系。原始肿瘤包含近单倍体细胞，有25或26条染色体，包括两条1号染色体、（7或7p+）和18号染色体。也有超二倍体（49 - 52条染色体）细胞，这些相同的三种染色体类型为四倍体；超二倍体细胞群体的双倍版本也可见。小鼠体内生长的肿瘤在第一次传代时的干系为26,X, +1, +7p+, +18，并且在十次连续体内传代过程中一直保持接近单倍体状态。生长稳定化已经出现，平均潜伏期不到3个月。这个可移植细胞系可用于评估人类巨细胞胶质母细胞瘤的化疗反应性以及研究人类实体瘤中的近单倍体现象。
