Chen Jia-Min, Cheng Yi-Ling, Yang Meng-Hui, Su Chen, Yu Hao
Fujian Key Laboratory of Oral Diseases and Fujian Provincial Engineering Research Center of Oral Biomaterial and Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.
Front Bioeng Biotechnol. 2024 Feb 8;12:1343329. doi: 10.3389/fbioe.2024.1343329. eCollection 2024.
Dental erosion and abrasion pose significant clinical challenges, often leading to exposed dentinal tubules and dentine demineralization. The aim of this study was to analyse the efficacy of quercetin-encapsulated hollow mesoporous silica nanocomposites (Q@HMSNs) on the prevention of dentine erosion and abrasion. Q@HMSNs were synthesized, characterized, and evaluated for their biocompatibility. A total of 130 dentine specimens (2 mm × 2 mm × 2 mm) were prepared and randomly distributed into 5 treatment groups (n = 26): DW (deionized water, negative control), NaF (12.3 mg/mL sodium fluoride, positive control), Q (300 μg/mL quercetin), HMSN (5.0 mg/mL HMSNs), and Q@HMSN (5.0 mg/mL Q@HMSNs). All groups were submitted to erosive (4 cycles/d) and abrasive (2 cycles/d) challenges for 7 days. The specimens in the DW, NaF, and Q groups were immersed in the respective solutions for 2 min, while treatment was performed for 30 s in the HMSN and Q@HMSN groups. Subsequently, the specimens were subjected to additional daily erosion/abrasion cycles for another 7 days. The effects of the materials on dentinal tubule occlusion and demineralized organic matrix (DOM) preservation were examined by scanning electron microscopy (SEM). The penetration depth of rhodamine B fluorescein into the etched dentine was assessed using confocal laser scanning microscopy (CLSM). The erosive dentine loss (EDL) and release of type I collagen telopeptide (ICTP) were measured. The data were analysed by one-way analysis of variance (ANOVA) with Tukey's test ( = 0.05). Q@HMSNs were successfully synthesized and showed minimal toxicity to human dental pulp stem cells (HDPSCs) and gingival fibroblasts (HGFs). Q@HMSNs effectively occluded the dentinal tubules, resulting in a thicker DOM in the Q@HMSN group. The CLSM images showed more superficial penetration in the HMSN and Q@HMSN groups than in the quercetin, NaF, and DW groups. The Q@HMSN group exhibited a significantly lower EDL and reduced ICTP levels compared to the other groups ( < 0.05). Q@HMSNs hold promise for inhibiting dentine erosion and abrasion by promoting tubule occlusion and DOM preservation.
牙齿侵蚀和磨损带来了重大的临床挑战，常常导致牙本质小管暴露和牙本质脱矿。本研究的目的是分析槲皮素包裹的中空介孔二氧化硅纳米复合材料（Q@HMSNs）对预防牙本质侵蚀和磨损的效果。合成了Q@HMSNs，对其进行了表征，并评估了其生物相容性。总共制备了130个牙本质标本（2毫米×2毫米×2毫米），并随机分为5个治疗组（n = 26）：DW（去离子水，阴性对照）、NaF（12.3毫克/毫升氟化钠，阳性对照）、Q（300微克/毫升槲皮素）、HMSN（5.0毫克/毫升HMSNs）和Q@HMSN（5.0毫克/毫升Q@HMSNs）。所有组均接受侵蚀（每天4个循环）和磨损（每天2个循环）挑战，持续7天。DW、NaF和Q组的标本在各自溶液中浸泡2分钟，而HMSN和Q@HMSN组的处理时间为30秒。随后，标本再进行另外7天的每日侵蚀/磨损循环。通过扫描电子显微镜（SEM）检查材料对牙本质小管闭塞和脱矿有机基质（DOM）保存的影响。使用共聚焦激光扫描显微镜（CLSM）评估罗丹明B荧光素渗入蚀刻牙本质的深度。测量侵蚀性牙本质损失（EDL）和I型胶原端肽（ICTP）的释放。数据通过单因素方差分析（ANOVA）和Tukey检验进行分析（α = 0.05）。Q@HMSNs成功合成，对人牙髓干细胞（HDPSCs）和牙龈成纤维细胞（HGFs）显示出最小的毒性。Q@HMSNs有效地闭塞了牙本质小管，导致Q@HMSN组的DOM更厚。CLSM图像显示，HMSN和Q@HMSN组的渗透比槲皮素、NaF和DW组更浅。与其他组相比，Q@HMSN组的EDL显著更低，ICTP水平降低（P < 0.05）。Q@HMSNs有望通过促进小管闭塞和DOM保存来抑制牙本质侵蚀和磨损。
