Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China; Department of Stomatology, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen 361006, China.
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, China.
Dent Mater. 2020 Nov;36(11):1430-1436. doi: 10.1016/j.dental.2020.08.013. Epub 2020 Sep 11.
The aim of the present study was to evaluate the effect of quercetin on the acid resistance of human dentin through both laboratory and clinical studies.
Two hundred and twelve dentin blocks (2 mm × 2 mm × 2 mm) were prepared and used. For the laboratory study, dentin specimens were randomly divided into 8 groups (n = 12): deionized water, ethanol, 1.23 × 10 μg/ml sodium fluoride (NaF), 120 μg/ml chlorhexidine, 183.2 μg/ml epigallocatechin gallate (EGCG), and 75 μg/ml, 150 μg/ml, and 300 μg/ml quercetin (Q75, Q150, and Q300). The specimens were treated with the respective solutions for 2 min and then subjected to in vitro erosion (4 cycles/d for 7 d). The surface microhardness loss (%SMH), erosive dentin wear, and surface morphology were evaluated and compared. For the impact on MMP inhibition, the release of crosslinked carboxyterminal telopeptide of type I collagen (ICTP) and the thickness of the demineralized organic matrix (DOM) were measured using additional dentin specimens. For the clinical study, the specimens were treated with NaF or Q300 for 2 min and then subjected to in vivo erosion (4 cycles/d for 7 d). The %SMH and erosive dentin wear of the specimens were measured to determine whether quercetin similarly inhibits erosion in situ.
The quercetin-treated group had a significantly lower %SMH and erosive dentin wear than any other group, and the effect was concentration-dependent in vitro (P < 0.05). Dentin treated with quercetin produced significantly less ICTP and had a thicker DOM than the control dentin (P < 0.05). After in vivo erosion, the %SMH and erosive dentin wear of the Q300 group were significantly lower than those of the control group (P < 0.05).
The application of quercetin was shown, for the first time, to increase the acid resistance of human dentin, possibly through MMP inhibition and DOM preservation.
本研究旨在通过实验室和临床研究评估槲皮素对人牙本质耐酸性能的影响。
制备 212 块牙本质块（2mm×2mm×2mm）。在实验室研究中，牙本质标本随机分为 8 组（n=12）：去离子水、乙醇、1.23×10μg/ml 氟化钠（NaF）、120μg/ml 洗必泰、183.2μg/ml 表没食子儿茶素没食子酸酯（EGCG）以及 75μg/ml、150μg/ml 和 300μg/ml 槲皮素（Q75、Q150 和 Q300）。将标本用相应溶液处理 2min，然后进行体外侵蚀（7d 内 4 个周期/d）。评估并比较表面显微硬度损失（%SMH）、侵蚀性牙本质磨损和表面形态。为了评估对 MMP 抑制的影响，使用额外的牙本质标本测量交联羧基末端肽型 I 胶原（ICTP）的释放和脱矿有机基质（DOM）的厚度。在临床研究中，将标本用 NaF 或 Q300 处理 2min，然后进行体内侵蚀（7d 内 4 个周期/d）。测量标本的%SMH 和侵蚀性牙本质磨损，以确定槲皮素是否同样可以抑制原位侵蚀。
与其他组相比，槲皮素处理组的%SMH 和侵蚀性牙本质磨损明显更低，体外呈浓度依赖性（P<0.05）。用槲皮素处理的牙本质产生的 ICTP 明显少于对照牙本质，DOM 较厚（P<0.05）。体内侵蚀后，Q300 组的%SMH 和侵蚀性牙本质磨损明显低于对照组（P<0.05）。
首次表明应用槲皮素可通过 MMP 抑制和 DOM 保存增加人牙本质的耐酸性。
