Kamirova Aina Maratovna, Sizova Elena Anatolyevna, Shoshin Daniil Evgenievich, Ivanishcheva Anastasia Pavlovna
Federal Research Centre for Biological Systems and Agricultural Technologies of the Russian Academy of Sciences, Orenburg, Russia.
Orenburg State University, Orenburg, Russia.
Vet World. 2024 Jan;17(1):189-196. doi: 10.14202/vetworld.2024.189-196. Epub 2024 Jan 22.
At present, detailed studies are being conducted to confirm the safety of the use of metal-containing ultrafine particles (UFP) in animal feeding, preventing the possibility of negative effects on productive qualities and physiological state, as well as on the environment and final consumer, that is, humans. Thus, the purpose of this research was to study the safety of cobalt- and manganese-containing UFP (UFP CoO, MnO UFP) together with (PB) herb extract in a bioluminescence inhibition test, as well as the effect of this composition on ruminal digestion .
The safety of the studied samples was determined using a multifunctional microplate analyzer TECAN Infinite F200 (Tecan Austria GmbH, Austria), recording the luminescence value of the bacterial strain (Ecolum, JSC NVO Immunotech, Russia). Dry matter (DM) digestibility studies were performed using the method on an "artificial rumen" model using an ANKOM Daisy II incubator unit (AD II; USA). The number of protozoa in ruminal fluid was counted in a Goryaev chamber. The bacterial mass was assessed by differential centrifugation followed by drying. This method is based on differences in the sedimentation rate of particles that differ in size and density.
UFP CoO and MnO at concentrations above 1.5 × 10 and 1.9 × 10 mol/L, respectively, have a pronounced bactericidal effect, suppressing more than 50% of the luminescence of . The combined use of UFP metals and plant extract increases the luminescence of the test object, indicating its safety. The combined use of UFP and PB increases the digestibility of feed DM and the number of protozoa in 1 mL of ruminal fluid; however, the combination of UFP MnO + PB (13.8%) yielded the best result, which is recommended for further research.
extract reduces the toxicity of UFP CoO and MnO , indicating that their combined use is safer.
目前，正在进行详细研究以确认动物饲料中使用含金属超细颗粒（UFP）的安全性，防止其对生产性能、生理状态以及环境和最终消费者（即人类）产生负面影响。因此，本研究的目的是在生物发光抑制试验中研究含钴和锰的UFP（UFP CoO、MnO UFP）与植物提取物（PB）的安全性，以及该组合物对瘤胃消化的影响。
使用多功能微孔板分析仪TECAN Infinite F200（奥地利Tecan Austria GmbH公司）测定所研究样品的安全性，记录细菌菌株（俄罗斯新西伯利亚免疫技术股份公司的Ecolum）的发光值。使用ANKOM Daisy II培养箱单元（AD II；美国）在“人工瘤胃”模型上采用该方法进行干物质（DM）消化率研究。在戈里亚耶夫计数板中对瘤胃液中原生动物的数量进行计数。通过差速离心然后干燥来评估细菌量。该方法基于大小和密度不同的颗粒沉降速率的差异。
UFP CoO和MnO浓度分别高于1.5×10和1.9×10 mol/L时，具有明显的杀菌作用，抑制超过50%的发光。UFP金属与植物提取物联合使用可增加测试对象的发光，表明其安全性。UFP与PB联合使用可提高饲料DM的消化率以及每毫升瘤胃液中原生动物的数量；然而，UFP MnO + PB（13.8%）的组合产生了最佳结果，建议进一步研究。
植物提取物降低了UFP CoO和MnO的毒性，表明它们联合使用更安全。
